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首次在中国新疆南部的蛹和成年绵羊虱（绵羊虱）中发现绵羊无浆体。

First report of Anaplasma ovis in pupal and adult Melophagus ovinus (sheep ked) collected in South Xinjiang, China.

机构信息

College of Animal Science, Tarim University, 705 Hongqiao South Road, Aral, 843300, People's Republic of China.

Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps, 705 Hongqiao South Road, Aral, 843300, People's Republic of China.

出版信息

Parasit Vectors. 2018 Apr 19;11(1):258. doi: 10.1186/s13071-018-2788-6.

DOI:10.1186/s13071-018-2788-6
PMID:29673387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5909264/
Abstract

BACKGROUND: Melophagus ovinus (sheep ked) is a blood-feeding ectoparasite that belongs to the family Hippoboscidae (Diptera: Hippoboscoidea) and mainly parasitizes sheep. The life-cycle of M. ovinus consists of three stages: larva, pupa and adult. It has a worldwide distribution and has been found in four provinces of China, especially South Xinjiang. In addition to causing direct damage to animal hosts, M. ovinus serves as a vector for disease transmission. In this study, our aim was to investigate the presence of Anaplasma spp. in pupal and adult M. ovinus. METHODS: A total of 93 specimens (including eight pupal specimens) of M. ovinus collected in South Xinjiang were selected for isolation of genomic DNA, followed by PCR amplification and sequencing of the msp4 gene of Anaplasma spp. The sequences were analyzed in MEGA 7.0 software and via online BLAST. RESULTS: PCR and sequencing results showed that all the specimens collected in 2013 were free of Anaplasma spp., whereas three and 25 specimens (including five pupal specimens) collected in 2016 and 2017, respectively, tested positive for Anaplasma spp. The analysis of 24 msp4 gene sequences (from four pupal specimens) confirmed the presence of A. ovis in M. ovinus specimens collected in South Xinjiang, China. The detected A. ovis isolates belong to Genotypes II and III. CONCLUSIONS: To the best of our knowledge, this is the first report of the detection of A. ovis DNA in pupal M. ovinus, confirming the vertical transmission of A. ovis in M. ovinus and the potential of M. ovinus to serve as a vector for A. ovis.

摘要

背景：绵羊虱蝇（绵羊虻）是一种吸血的外寄生虫，属于虻科（双翅目：虻科），主要寄生在绵羊身上。绵羊虱蝇的生命周期包括三个阶段：幼虫、蛹和成虫。它分布广泛，在中国四个省份都有发现，特别是在新疆南部。除了对动物宿主造成直接损害外，绵羊虱蝇还是疾病传播的媒介。在这项研究中，我们的目的是调查蛹和成虫绵羊虱蝇中是否存在无形体属。

方法：从新疆南部共采集了 93 只（包括 8 只蛹）绵羊虱蝇标本，用于分离基因组 DNA，然后对无形体属的 msp4 基因进行 PCR 扩增和测序。序列在 MEGA 7.0 软件和在线 BLAST 中进行分析。

结果：PCR 和测序结果显示，2013 年采集的所有标本均未检出无形体属，而 2016 年和 2017 年分别采集的 3 只和 25 只（包括 5 只蛹）标本检测出无形体属阳性。对 24 个 msp4 基因序列（来自 4 只蛹标本）的分析证实，在中国新疆南部采集的绵羊虱蝇标本中存在绵羊无形体。检测到的绵羊无形体分离株属于基因型 II 和 III。

结论：据我们所知，这是首次报道在蛹期绵羊虱蝇中检测到绵羊无形体 DNA，证实了绵羊无形体在绵羊虱蝇中的垂直传播以及绵羊虱蝇作为绵羊无形体传播媒介的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14f2/5909264/1e89b61e037e/13071_2018_2788_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14f2/5909264/b7e2a74bfda3/13071_2018_2788_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14f2/5909264/72e88f20b07e/13071_2018_2788_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14f2/5909264/1e89b61e037e/13071_2018_2788_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14f2/5909264/b7e2a74bfda3/13071_2018_2788_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14f2/5909264/72e88f20b07e/13071_2018_2788_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14f2/5909264/1e89b61e037e/13071_2018_2788_Fig3_HTML.jpg

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本文引用的文献

[1]
Occurrence of four Anaplasma species with veterinary and public health significance in sheep, northwestern China.

Ticks Tick Borne Dis. 2017-10-12

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Parasit Vectors. 2015-3-21

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Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China.

Parasit Vectors. 2015-2-19

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