Cui Yanyan, Yan Yaqun, Wang Xiaoxing, Cao Shuxuan, Zhang Yan, Jian Fuchun, Zhang Longxian, Wang Rongjun, Shi Ke, Ning Changshen
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Ticks Tick Borne Dis. 2017 Feb;8(2):283-289. doi: 10.1016/j.ttbdis.2016.12.001. Epub 2016 Dec 6.
In recent years, tick-borne diseases like anaplasmosis have become widespread worldwide threatening the health of both human and animals. Dogs play an important role in the epidemiology of several zoonotic tick-borne pathogens by acting as reservoirs. In this study, the status of Anaplasma phagocytophilum, A. platys, A. bovis and A. ovis infection were assessed in dogs in Henan, China, with PCR and phylogenetic analyses. Nested PCRs on 243 blood samples collected from dogs from different sampling sites revealed that thirty-three (13.6%) dogs were positive for one or more pathogens. The prevalence of Anaplasma spp. in stray dogs was 40.7% (24/59), which was much higher than that of pet dogs (4.0%, 7/175). The prevalence for A. ovis, A. bovis and A. phagocytophilum was 6.2%, 4.1% and 0.4%, respectively and mixed-infection of these three pathogens was found in only one stray dog (prevalence, 0.4%). None of the dogs was positive for A. platys. Phylogenetic analyses classified A. phagocytophilum into two distinct groups (East Asia and south Africa group, Europe and America group), whereas A. ovis and A. bovis showed a general classification into two groups (cluster 1 and cluster 2), respectively. The isolate (KX190783) of A. ovis from a stray dog fell in a clade with a human isolate from Cyprus (FJ460443) and shared 99.8% similarity with it. To the best of our knowledge, this study is the first report to identify A. bovis and A. ovis DNA in dogs in China and the mixed-infection of the three Anaplasma spp. (A. phagocytophilum, A. bovis and A. ovis) in dogs.
近年来，诸如无形体病等蜱传疾病在全球范围内广泛传播，威胁着人类和动物的健康。犬类作为储存宿主，在几种人兽共患蜱传病原体的流行病学中发挥着重要作用。在本研究中，采用聚合酶链反应（PCR）和系统发育分析方法，对中国河南省犬类中嗜吞噬细胞无形体、血小板无形体、牛无形体和绵羊无形体的感染状况进行了评估。对从不同采样地点采集的243份犬血样进行巢式PCR检测，结果显示，33只（13.6%）犬感染了一种或多种病原体。流浪犬中无形体属的感染率为40.7%（24/59），远高于宠物犬（4.0%，7/175）。绵羊无形体、牛无形体和嗜吞噬细胞无形体的感染率分别为6.2%、4.1%和0.4%，仅在一只流浪犬中发现这三种病原体的混合感染（感染率为0.4%）。所有犬类均未检测到血小板无形体阳性。系统发育分析将嗜吞噬细胞无形体分为两个不同的组（东亚和南非组、欧洲和美洲组），而绵羊无形体和牛无形体分别大致分为两组（簇1和簇2）。一只流浪犬的绵羊无形体分离株（KX190783）与来自塞浦路斯的人类分离株（FJ460443）归为一个进化枝，相似度为99.8%。据我们所知，本研究首次在中国犬类中鉴定出牛无形体和绵羊无形体DNA以及三种无形体属（嗜吞噬细胞无形体、牛无形体和绵羊无形体）在犬类中的混合感染。
