State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Food Funct. 2020 Mar 26;11(3):2693-2703. doi: 10.1039/c9fo02975c.
Broadly, bok-choy is known for its potential benefits as part of a human diet. However, the effects and deeper investigations of bok-choy on human health are still insufficient. This study aimed to investigate the beneficial effects of two cultivars of bok-choy, 'Suzhouqing' (green cultivar) and 'Ziluolan' (purple cultivar), on growth performance, lipid metabolism and related gene expressions in Syrian golden hamsters. Fifty six male Syrian golden hamsters (6-months-old) were randomly assigned into 6 groups: normal diet (A), high-fat diet (B), high-fat diet + 5% 'Suzhouqing' (C), high-fat diet + 7% 'Suzhouqing' (D), high-fat diet + 5% 'Ziluolan' (E), and high-fat diet + 7% 'Ziluolan' (F), fed for 56 consecutive days. On day 0, 28 and 56, blood and liver samples were collected to examine the lipid profile, liver enzymes, histomorphology and related gene expressions. The results showed that group B had significantly increased levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase, while (P < 0.05) showed impaired levels of high-density lipoprotein cholesterol compared with group A. Group D, E and F had significantly reduced levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase, while the level of high-density lipoprotein cholesterol was significantly increased compared with group B. Remarkably, the mRNA expressions of CEBP-α, DGAT1, lipoprotein lipase (LPL), FASN and 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA) were significantly up-regulated and carnitine palmitoyl transferase 2 (CPT2), Cyp27A1 and proliferator activated receptor alpha (PPAR-α) were significantly down-regulated in group B compared with group A. However, in group D, E and F, the mRNA expression levels of CCAAT enhancer binding protein alpha, DGAT1, LPL, FASN and HMG-CoA were significantly down-regulated and CPT2, Cyp27A1 and PPAR-α were significantly up-regulated compared with group B. In conclusion, different amounts of bok-choy added to the diets incredibly improved the lipid-profile, enhanced liver enzyme activities and related gene expression. The hamsters supplemented with 7% 'Ziluolan' exhibited the best performance among all the other high-fat groups, which shows that Ziluolan could be a great alternative for the reduction of fat accumulation and conserving health.
广泛而言，白菜被认为对人类饮食有潜在益处。然而，白菜对人类健康的影响和更深入的研究仍然不足。本研究旨在探究两种白菜品种（绿叶品种“苏州青”和紫叶品种“紫露兰”）对叙利亚金黄仓鼠生长性能、脂代谢及相关基因表达的有益作用。56 只 6 月龄雄性叙利亚金黄仓鼠随机分为 6 组：正常饮食组（A）、高脂饮食组（B）、高脂饮食+5%“苏州青”组（C）、高脂饮食+7%“苏州青”组（D）、高脂饮食+5%“紫露兰”组（E）和高脂饮食+7%“紫露兰”组（F），连续喂养 56 天。于第 0、28 和 56 天采集血样和肝脏样本，检测血脂谱、肝酶、组织形态学和相关基因表达。结果表明，B 组总胆固醇、甘油三酯、低密度脂蛋白胆固醇、丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶水平显著升高（P<0.05），高密度脂蛋白胆固醇水平显著降低（P<0.05）。与 B 组相比，D、E 和 F 组总胆固醇、甘油三酯、低密度脂蛋白胆固醇、丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶水平显著降低，高密度脂蛋白胆固醇水平显著升高。值得注意的是，与 A 组相比，B 组 CEBP-α、DGAT1、脂蛋白脂肪酶（LPL）、脂肪酸合酶（FASN）和 3-羟-3-甲基戊二酰辅酶 A 还原酶（HMG-CoA）mRNA 表达显著上调，肉碱棕榈酰转移酶 2（CPT2）、CYP27A1 和过氧化物酶体增殖物激活受体α（PPAR-α）mRNA 表达显著下调。然而，与 B 组相比，D、E 和 F 组 CCAAT 增强子结合蛋白-α、DGAT1、LPL、FASN 和 HMG-CoA 的 mRNA 表达水平显著下调，CPT2、CYP27A1 和 PPAR-α的 mRNA 表达水平显著上调。综上所述，不同添加量的白菜添加到饮食中，可显著改善血脂谱，增强肝酶活性及相关基因表达。添加 7%“紫露兰”的仓鼠在所有高脂组中表现最佳，这表明“紫露兰”可作为减少脂肪积累和保持健康的良好替代品。
