Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain.
Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain; Institut Mediterrani d'Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain.
Syst Appl Microbiol. 2018 Jul;41(4):340-347. doi: 10.1016/j.syapm.2018.03.008. Epub 2018 Apr 11.
Strains V113, V92 and V120 have been isolated from sand samples taken at the Atlantic intertidal shore in Galicia, Spain, after the Prestige oil spill. A preliminary analysis of the 16S rRNA and the partial rpoD gene sequences indicated that these strains belonged to the Pseudomonas genus, but they were distinct from any known Pseudomonas species. They were extensively characterized by a polyphasic taxonomic approach and phylogenetic data that confirmed that these strains belonged to the Pseudomonas pertucinogena group. Phylogenetic analysis of 16S rRNA, gyrB and rpoD gene sequences showed that the three strains were 99% similar and were closely related to members of the P. pertucinogena group, with less than 94% similarity to strains of established species; Pseudomonas pachastrellae was the closest relative. The Average Nucleotide Index based on blast values was 89.0% between V113 and the P. pachastrellae type strain, below the accepted species level (95%). The predominant cellular fatty acid contents and whole cell protein profiles determined by MALDI-TOF mass spectrometry also differentiated the studied strains from known Pseudomonas species. We therefore conclude that strains V113, V92 and V120 represent a novel species of Pseudomonas, for which the name Pseudomonas gallaeciensis is proposed; the type strain is V113 (=CCUG 67583=LMG 29038).
菌株 V113、V92 和 V120 是从西班牙加利西亚大西洋潮间带沙样中分离出来的，这些沙样是在“威望号”溢油事件之后采集的。16S rRNA 和部分 rpoD 基因序列的初步分析表明，这些菌株属于假单胞菌属，但与任何已知的假单胞菌属物种都不同。通过多相分类学方法和系统发育数据分析对它们进行了广泛的鉴定，这些数据证实这些菌株属于假单胞菌属 pertucinogena 群。16S rRNA、gyrB 和 rpoD 基因序列的系统发育分析表明，这 3 个菌株的相似度为 99%，与 pertucinogena 群的成员密切相关，与已建立的种的菌株相似度低于 94%；与假单胞菌属 pachastrellae 的相似度最高。基于 blast 值的平均核苷酸指数在菌株 V113 和假单胞菌属 pachastrellae 模式菌株之间为 89.0%，低于公认的种水平（95%）。通过 MALDI-TOF 质谱法确定的主要细胞脂肪酸含量和全细胞蛋白图谱也将研究菌株与已知的假单胞菌属物种区分开来。因此，我们得出结论，菌株 V113、V92 和 V120 代表了一种新型的假单胞菌属，我们提议将其命名为假单胞菌属 gallaeciensis；模式菌株为 V113（=CCUG 67583=LMG 29038）。
