See-Too Wah Seng, Salazar Sergio, Ee Robson, Convey Peter, Chan Kok-Gan, Peix Álvaro
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science University of Malaya, 50603 Kuala Lumpur, Malaysia; National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain.
Syst Appl Microbiol. 2017 Jun;40(4):191-198. doi: 10.1016/j.syapm.2017.03.002. Epub 2017 Apr 18.
In this study we analysed three bacterial strains coded L10.10, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring in Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993. Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95% with respect to the closest related species of Pseudomonas, confirming its phylogenetic affiliation. The ANI values were less than 86% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/ C16:1 ω6c in summed feature 3 and C18:1 ω7c / C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. Growth occurs at 4-30°C, and at pH 4.0-10. The DNA G+C content is 58.2-58.3mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10 (LMG 29628, DSM 101070).
在本研究中，我们分析了三个编码为L10.10、A4R1.5和A4R1.12的细菌菌株，这些菌株是在对南极土壤中群体淬灭细菌的研究过程中分离得到的。这三个菌株的16S rRNA基因序列相同，与最接近的相关物种魏氏假单胞菌WS4993的序列显示出99.7%的成对相似性。因此，这三个菌株被归类为假单胞菌属。看家基因（rpoB、rpoD和gyrB）序列分析显示，与最接近的假单胞菌相关物种的相似性为84 - 95%，证实了其系统发育归属。与最接近的相关物种模式菌株的ANI值小于86%。呼吸醌为Q9。主要脂肪酸为特征性总和3中的C16:0、C16:1 ω7c/C16:1 ω6c以及特征性总和8中的C18:1 ω7c/C18:1 ω6c。这些菌株氧化酶和过氧化氢酶呈阳性。在4 - 30°C和pH 4.0 - 10的条件下生长。DNA G + C含量为58.2 - 58.3mol%。综合的基因型、表型和化学分类学数据支持将菌株L10.10、A4R1.5和A4R1.12分类为假单胞菌属的一个新物种，为此提出名称为P. versuta sp. nov.。模式菌株为L10.10（LMG 29628，DSM 101070）。
