Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; Beijing National Laboratory for Molecular Science, Beijing 100190, China.
Talanta. 2018 Jul 1;184:468-474. doi: 10.1016/j.talanta.2018.03.040. Epub 2018 Mar 15.
The signal amplification methods of surface plasmon resonance (SPR) are mainly with sample selectivity. Herein proposed is a liquid core coupling SPR sensing method with adjustable sensitivity and universal applicability. A liquid core coupling unit and based on which a wavelength interrogation SPR sensor have been designed and constructed, in which a conventional chip could be used with an ingenious design and the sensitivity can be adjusted by changing the liquid core refractive index. The experimental data revealed that the sensitivity can be enhanced for 7.8 times by decreasing the liquid core refractive index from 1.5174 to 1.4502. The real applicability was validated by determination of carcinoembryonic antigen (CEA) with the signal intensity of the early stage colon cancer patient clearly up-regulated for nearly 18 folds compared with the healthy. It exhibited an adequate sensitivity, and further considering its simplicity avoiding the troublesome analysis process and/or chip preparing procedure, and extendibility to SPR imaging, the proposed method would be promising for medical diagnostics, food safety, environmental monitoring, and so on.
表面等离子体共振（SPR）的信号放大方法主要具有样品选择性。本文提出了一种可调灵敏度和普遍适用性的液芯耦合 SPR 传感方法。设计并构建了一种液芯耦合单元和基于该单元的波长询问 SPR 传感器，其中可以巧妙的设计使用常规芯片，并通过改变液芯折射率来调整灵敏度。实验数据表明，通过将液芯折射率从 1.5174 降低到 1.4502，灵敏度可增强 7.8 倍。通过对早期结肠癌患者的信号强度进行检测，验证了其实用性，与健康者相比，信号强度明显上调了近 18 倍。该方法具有足够的灵敏度，并且进一步考虑到其简单性，避免了繁琐的分析过程和/或芯片制备过程，以及向 SPR 成像的可扩展性，该方法有望在医学诊断、食品安全、环境监测等领域得到应用。
