Zhang Jinjun, Du Yaying, Zhang Xiaoxue, Li Mengchen, Li Xingrui
Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
J Cancer. 2018 Mar 26;9(7):1318-1328. doi: 10.7150/jca.20150. eCollection 2018.
Recent evidence indicates that long non-coding RNAs play important roles in tumorigenesis and cancer progression. -activated non-protein coding RNA () is a novel and potential regulator of cancer cell proliferation and migration. However, little is known regarding the role of in papillary thyroid cancer (PTC). The current study used quantitative PCR to demonstrate that was significantly downregulated in 60 paired PTC tissues compared with normal tissues. In addition, was significantly correlated with lymph node metastasis ( = 0.02). Furthermore, Cell Counting Kits and Transwell assays were used to demonstrate that knocking down with short hairpin RNA (shRNA) transfection significantly promoted the proliferation and invasion of PTC cell lines. The flow cytometric analysis of apoptosis and the cell cycle revealed that the overexpression of inhibited cancer cell proliferation and invasion, which was associated with the induction of cell-cycle G2/M phase arrest and increased apoptosis. Moreover, western blotting was used to show that the MAPK and PI3K-Akt pathways were aberrantly activated during -mediated PTC cell proliferation and migration. These findings revealed that functions as a tumor suppressor during thyroid carcinogenesis.
最近的证据表明，长链非编码RNA在肿瘤发生和癌症进展中发挥重要作用。-激活的非蛋白质编码RNA（）是癌细胞增殖和迁移的一种新型潜在调节因子。然而，关于在甲状腺乳头状癌（PTC）中的作用知之甚少。当前研究使用定量PCR证明，与正常组织相比，在60对PTC组织中显著下调。此外，与淋巴结转移显著相关（=0.02）。此外，使用细胞计数试剂盒和Transwell试验证明，用短发夹RNA（shRNA）转染敲低可显著促进PTC细胞系的增殖和侵袭。凋亡和细胞周期的流式细胞术分析显示，的过表达抑制癌细胞增殖和侵袭，这与诱导细胞周期G2/M期阻滞和增加凋亡有关。此外，蛋白质免疫印迹法用于显示在介导的PTC细胞增殖和迁移过程中，MAPK和PI3K-Akt信号通路被异常激活。这些发现揭示了在甲状腺癌发生过程中作为一种肿瘤抑制因子发挥作用。
