Li Jing, Wang Jinfeng, Zhou Wei, Zhang Shuqin, Le Yuan, He Rong
Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Department of Pediatrics, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China.
Oncol Lett. 2017 Oct;14(4):4751-4757. doi: 10.3892/ol.2017.6770. Epub 2017 Aug 18.
The long non-coding RNA BRAF-activated non-coding RNA (BANCR) has been reported to serve essential roles in the progress of a various cancer types. The purpose of the present study was to investigate the effect of lncRNA BANCR in HCC cells. The expression of BANCR in the HCC cell line Huh7 and a normal liver cell line were determined using reverse transcription-quantitative polymerase chain reaction. The expression of BANCR in Huh7 cells was downregulated by short hairpin (sh)RNA. Subsequently, the proliferation, apoptosis, migration and invasion rates were determined, along with the activity of MAPK/ERK kinase (MEK) and mitogen-activated protein kinase signaling pathways. The results revealed that BANCR was overexpressed in Huh7 cells when compared with normal liver cells. The downregulation of BANCR significantly inhibited the proliferation and colony formation ability, and induced cell cycle arrest and apoptosis of Huh7 cells. The migration and invasion of Huh7 cells were also suppressed in BANCR shRNA-transfected cells. The downregulation of BANCR significantly inhibited the activity of MEK, extracellular signal-regulated kinase and janus kinase signaling pathways. Collectively, these findings demonstrated that the lncRNA BANCR is oncogenic in Huh7 cells, and may be a promising molecular target for HCC therapy.
据报道，长链非编码RNA BRAF激活的非编码RNA（BANCR）在多种癌症类型的进展中发挥重要作用。本研究的目的是探讨lncRNA BANCR在肝癌细胞中的作用。使用逆转录定量聚合酶链反应测定BANCR在肝癌细胞系Huh7和正常肝细胞系中的表达。通过短发夹（sh）RNA下调Huh7细胞中BANCR的表达。随后，测定细胞的增殖、凋亡、迁移和侵袭率，以及丝裂原活化蛋白激酶/细胞外信号调节激酶（MEK）和丝裂原活化蛋白激酶信号通路的活性。结果显示，与正常肝细胞相比，Huh7细胞中BANCR过表达。BANCR的下调显著抑制了Huh7细胞的增殖和集落形成能力，并诱导细胞周期停滞和凋亡。在转染BANCR shRNA的细胞中，Huh7细胞的迁移和侵袭也受到抑制。BANCR的下调显著抑制了MEK、细胞外信号调节激酶和janus激酶信号通路的活性。总之，这些发现表明lncRNA BANCR在Huh7细胞中具有致癌作用，可能是肝癌治疗的一个有前景的分子靶点。
