Sameni Hamid Reza, Javadinia Sara Sadat, Safari Manouchehr, Tabrizi Amjad Mohammad Hasan, Khanmohammadi Nasrin, Parsaie Houman, Zarbakhsh Sam
Research Center of Nervous System Stem Cells, Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Int J Reprod Biomed. 2018 Feb;16(2):101-108.
Quercetin is a flavonoid with the ability to improve the growth of embryos in vitro, and actinomycin D is an inducer of apoptosis in embryonic cells.
The aim was to evaluate the effect of quercetin on the number of viable and apoptotic cells, the zona pellucida (ZP) thickness and the hatching rate of preimplantation embryos exposed to actinomycin D in mice.
Two-cell embryos were randomly divided into four groups (Control, Quercetin, actinomycin D, and Quercetin + actinomycin D group). Blastocysts percentage, hatched blastocysts, and ZP thickness of blastocysts was measured. The number of blastomeres was counted by Hoechst and propidium iodide staining and the apoptotic cells number was counted by TUNEL assay.
The results showed that the use of quercetin significantly improved the growth of embryos compared to the control group (p=0.037). Moreover, quercetin reduced the destructive effects of actinomycin D on the growth of embryos significantly (p=0.026).
quercetin may protect the embryos against actinomycin D so that increases the number of viable cells and decreases the number of apoptotic cells, which can help the expansion of the blastocysts, thinning of the ZP thickness and increasing the hatching rate in mouse embryos.
槲皮素是一种黄酮类化合物，具有促进胚胎体外生长的能力，而放线菌素D是胚胎细胞凋亡的诱导剂。
旨在评估槲皮素对暴露于放线菌素D的小鼠植入前胚胎的活细胞和凋亡细胞数量、透明带（ZP）厚度及孵化率的影响。
将二细胞胚胎随机分为四组（对照组、槲皮素组、放线菌素D组和槲皮素+放线菌素D组）。测量囊胚百分比、孵化囊胚及囊胚的ZP厚度。通过Hoechst和碘化丙啶染色计数卵裂球数量，通过TUNEL法计数凋亡细胞数量。
结果显示，与对照组相比，使用槲皮素显著促进了胚胎生长（p=0.037）。此外，槲皮素显著降低了放线菌素D对胚胎生长的破坏作用（p=0.026）。
槲皮素可能保护胚胎免受放线菌素D的影响，从而增加活细胞数量，减少凋亡细胞数量，这有助于小鼠胚胎囊胚的扩张、ZP厚度变薄及孵化率提高。
