Mittal Mayank, Biswas Sanjay Kumar, Singh Vandana, Arela Nidhi, Katoch Vishwa Mohan, Das Ram, Yadav Virendra Singh, Bajaj Bharat, Mohanty Keshar Kunja
Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Taj Ganj, Agra, 282004, India.
Molecular Biology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Agra, 282004, India.
Mol Biol Rep. 2018 Aug;45(4):469-476. doi: 10.1007/s11033-018-4182-z. Epub 2018 Apr 19.
Tuberculosis (TB) is a disease of global importance. There is an increasing recognition of the role of Toll like receptors, important pattern recognition receptors of host immune system, in determining the susceptibility or resistance to TB in various populations. In an attempt to examine the importance of Toll like receptors in immune response to Mycobacterium tuberculosis infection, we explored two variants each of TLR2 and TLR9 in a population residing in Uttar Pradesh, India. Genotyping was performed to detect -196 to -174 del polymorphism and G2258A SNP (Arg753Gln, rs5743708) in TLR2 gene and -T1237C (rs5743836) and G2848A (rs352140) SNP in TLR9 gene in patients with pulmonary TB and healthy controls. The A allele of G2848A SNP in TLR9 gene was found with a marginally higher frequency among TB patients as compared to healthy controls, suggesting that A allele at position 2848 of TLR9 gene may be associated with susceptibility to TB in North Indian population [p = 0.05, Mantel-Haenszel OR = 1.34, 95% CI (1.0-1.82)].
结核病（TB）是一种具有全球重要性的疾病。宿主免疫系统重要的模式识别受体——Toll样受体在决定不同人群对结核病的易感性或抵抗力方面所起的作用，正日益受到认可。为了研究Toll样受体在对结核分枝杆菌感染的免疫反应中的重要性，我们在印度北方邦的一个人群中探究了TLR2和TLR9各自的两个变体。对肺结核患者和健康对照者进行基因分型，以检测TLR2基因中的-196至-174缺失多态性和G2258A单核苷酸多态性（Arg753Gln，rs5743708），以及TLR9基因中的-T1237C（rs5743836）和G2848A（rs352140）单核苷酸多态性。与健康对照者相比，在肺结核患者中发现TLR9基因G2848A单核苷酸多态性的A等位基因频率略高一些，这表明TLR9基因第2848位的A等位基因可能与印度北方人群对结核病的易感性相关[p = 0.05，Mantel-Haenszel比值比 = 1.34，95%可信区间（1.0 - 1.82）]。
