Wu Man-Jun, Guo Qiao-Sheng, Shi Hong-Zhuan, Liu Fei, Shi Guo-Wei, Wu Bin
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2018 Mar;43(5):934-937. doi: 10.19540/j.cnki.cjcmm.20180109.002.
To study the effects of ingestion on antithrombin activities in different tissues of Whitmania pigra, the salivary glands, ingluvies, intestine and muscle of adult leeches were weighted on the 1st d, 3rd d, 5th d, 7th d and 11th d after feeding, respectively, and meanwhile antithrombin activity was measured by antithrombin titration method. The results showed that the antithrombin activity of salivary glands in different stages was significantly higher than that in other tissues (<0.05). The activity of antithrombin in muscle tissue increased initially and then decreased with the prolongation of the time after feeding, and the peak was observed on the 5th day after feeding (<0.05). The activity of antithrombin in the salivary glands, gluvies and intestine were found the highest on the 1st day after feeding(<0.05), and then gradually decreased with the prolongation of the time of stopping the diet. The total amount of antithrombin activity on the 5th day was increased by 49.5%, 73.5%, 69.1% and 126.0% comparing with the 1st, 3rd, 7th and 11th day after feeding, respectively (<0.05). In summary, both the feeding behavior and the food can induce the secretion of anticoagulant substances in the salivary glands and the digestive tract. The total amount of antithrombin activity was the highest on the 5th day after feeding and the 5th day after feeding was suggested as harvesting time.
为研究摄食对宽体金线蛭不同组织抗凝血酶活性的影响，分别于喂食后第1天、第3天、第5天、第7天和第11天对成年水蛭的唾液腺、嗉囊、肠道和肌肉进行称重，同时采用抗凝血酶滴定法测定抗凝血酶活性。结果显示，不同阶段唾液腺的抗凝血酶活性显著高于其他组织（<0.05）。肌肉组织中抗凝血酶活性随喂食后时间延长先升高后降低，在喂食后第5天达到峰值（<0.05）。唾液腺、嗉囊和肠道中的抗凝血酶活性在喂食后第1天最高（<0.05），随后随着停食时间延长逐渐降低。与喂食后第1天、第3天、第7天和第11天相比，第5天抗凝血酶活性总量分别增加了49.5%、73.5%、69.1%和126.0%（<0.05）。综上所述，摄食行为和食物均可诱导唾液腺和消化道分泌抗凝物质。喂食后第5天抗凝血酶活性总量最高，建议以喂食后第5天作为采收时间。
