Kwiatkowska-Patzer B, Zalewska T
Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
Basic Res Cardiol. 1988 Jan-Feb;83(1):43-7. doi: 10.1007/BF01907103.
The aim of the study was to investigate the effect of propranolol upon protein synthesis and degradation processes in cell-free subfractions of rat myocardium in experimental cardiac hypertrophy induced by aortic stenosis. It was found that hypertrophy stimulates incorporation of 3H-amino acids by the postmitochondrial supernatant (PMS) by 17 +/- 4% (mean +/- SE). Propranolol 10 6M inhibited protein synthesis in the control and experimental groups by 37 +/- 5% and 34 +/- 7%, respectively. At a concentration of 5 X 10 6M, propranolol-induced inhibition in each group was 74 +/- 4% and 58 +/- 5%, respectively. The activity of neutral proteases in PMS of hypertrophic hearts increased up to 255 +/- 29 IU in comparison to 154 +/- 10 IU in controls; in the cytosol it was unchanged. Propranolol had no effect upon the activity of proteases in both fractions. Activity of calcium-activated neutral proteases (CANP) remained at the same level in control and hypertrophic hearts and was unaffected by propranolol.
本研究的目的是探讨普萘洛尔对主动脉狭窄诱导的实验性心肌肥大大鼠心肌无细胞亚组分中蛋白质合成和降解过程的影响。结果发现，肥大可使线粒体后上清液（PMS）中3H-氨基酸的掺入量增加17±4%（平均值±标准误）。10^6M的普萘洛尔分别使对照组和实验组的蛋白质合成抑制了37±5%和34±7%。在5×10^6M的浓度下，普萘洛尔在每组中诱导的抑制率分别为74±4%和58±5%。与对照组的154±10IU相比，肥大心脏PMS中中性蛋白酶的活性增加至255±29IU；在细胞溶质中则无变化。普萘洛尔对这两个组分中的蛋白酶活性均无影响。钙激活中性蛋白酶（CANP）的活性在对照心脏和肥大心脏中保持在相同水平，且不受普萘洛尔的影响。
