心肌肥大时胞质溶胶对体外RNA转运的影响。
Effect of cytosol on transport of RNA in vitro during cardiac hypertrophy.
作者信息
Meenakshi S, Thirunavukkarasu C, Rajamanickam C
出版信息
Biochem J. 1983 Feb 1;209(2):285-90. doi: 10.1042/bj2090285.
An enhanced RNA-transport activity was observed in vitro from nuclei obtained from animals with cardiac hypertrophy as compared with that of sham-operated controls. The 100 000 g supernatants obtained from hypertrophic hearts stimulated the RNA transport from nuclei of sham-operated controls, and this stimulation was maximum with 40% supernatant. Ca2+- and nucleic acid-dependent ATPase and alkaline phosphatase activities, which may be involved in an energy-dependent transport, were high in nuclei from hypertrophic hearts, and the nuclei of sham-operated animals showed higher activities of these enzymes after incubation with supernatant from hypertrophic hearts, which stimulates the RNA transport in vitro from nuclei of sham-operated animals.
与假手术对照组相比，在体外观察到从患有心肌肥大的动物获得的细胞核具有增强的RNA转运活性。从肥大心脏获得的100000g上清液刺激了假手术对照组细胞核的RNA转运，且40%的上清液刺激作用最大。可能参与能量依赖性转运的Ca2+和核酸依赖性ATP酶以及碱性磷酸酶活性，在肥大心脏的细胞核中较高，并且假手术动物的细胞核在与肥大心脏的上清液孵育后，这些酶的活性更高，该上清液在体外刺激了假手术动物细胞核的RNA转运。
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