State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, China.
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Vet Microbiol. 2024 Jan;288:109947. doi: 10.1016/j.vetmic.2023.109947. Epub 2023 Dec 13.
Rotavirus B (RVB) is a causative agent leading to acute viral gastroenteritis diarrhea in both children and young animals, and has been commonly detected in piglets. In order to determine the causative agent of diarrheal outbreak occurring in December 2022 in piglets from a pig herd in Luoyang, Henan province of China, four common viral pathogens causing piglet diarrhea-three coronaviruses and rotavirus A (RVA) were first tested and found negative, therefore metagenomic sequencing was performed to explore other potential pathogens in the diarrheal samples. Unexpectedly, the most abundant viral reads mapped to RVB, and were de novo assembled to complete 11 viral gene segments. Sequence comparisons revealed that 5 gene segments encoding VP1, VP2, VP3, NSP3 and NSP4 of RVB strain designated as HNLY-2022 are most closely related to RVB strains derived from herbivores with low nucleotide similarities of 65.7-75.3%, and the remaining segments were relatively close to porcine RVB strains with the VP4 gene segment showing very low nucleotide identity (65.0%) with reference strains, indicating HNLY-2022 is a new reassortant RVB strain. Based on the previously proposed genotype classification criterion, the genotype constellation of RVB strain HNLY-2022 is G6-P[6]-I4-R6-C6-M6-A7-N5-T7-E5-H4 with more than half of the genotypes (P[6], R6, C6, M6, T7 and E5) newly reported. Therefore, the new reassortant RVB strain is the likely causative agent for the diarrheal outbreak of piglets occurred in China and more epidemiological studies should be conducted to monitor the spread of this newly identified porcine RVB strain.
轮状病毒 B (RVB) 是导致儿童和幼小动物急性病毒性肠胃炎腹泻的病原体，已在仔猪中普遍检测到。为了确定 2022 年 12 月河南省洛阳市某猪场仔猪腹泻爆发的病原体，首先检测了导致仔猪腹泻的四种常见病毒病原体——三种冠状病毒和轮状病毒 A (RVA)，结果均为阴性，因此进行了宏基因组测序以探索腹泻样本中的其他潜在病原体。出乎意料的是，最丰富的病毒读段与 RVB 相对应，并从头组装完成了 11 个病毒基因片段。序列比较显示，编码 RVB 株 HNLY-2022 的 VP1、VP2、VP3、NSP3 和 NSP4 的 5 个基因片段与源自草食动物的 RVB 株的亲缘关系最密切，核苷酸相似性为 65.7-75.3%，其余片段与猪 RVB 株相对接近，VP4 基因片段与参考株的核苷酸同一性非常低（65.0%），表明 HNLY-2022 是一种新的重组 RVB 株。根据先前提出的基因型分类标准，RVB 株 HNLY-2022 的基因型组合为 G6-P[6]-I4-R6-C6-M6-A7-N5-T7-E5-H4，超过一半的基因型（P[6]、R6、C6、M6、T7 和 E5）是新报道的。因此，这种新的重组 RVB 株可能是导致中国仔猪腹泻爆发的病原体，应进行更多的流行病学研究来监测这种新鉴定的猪 RVB 株的传播。
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