Department of Ophthalmology, Hanusch Hospital, Vienna, Austria.
Clinical Department of Laboratory Medicine, Medical University of Vienna, Austria.
Invest Ophthalmol Vis Sci. 2018 Apr 1;59(5):1855-1860. doi: 10.1167/iovs.18-23963.
To determine the pharmacokinetics of perorally administered caffeine, a widely consumed and potent dietary antioxidant, in the anterior lens capsule and lens epithelial cells, a crucial cell monolayer for cataract development.
Bilateral cataract patients were scheduled for cataract surgery with a caffeine abstinence of 1 week before surgery of each eye. At the day of surgery of the second eye patients were administered no drink (0-mg group) or coffee with 60-, 120-, or 180-mg caffeine. After capsulorhexis the lens capsule including lens epithelial cells was transferred to a test tube for analysis of caffeine concentration by gas chromatography-mass spectrometry (GC-MS/MS).
Coffee consumption significantly (P < 0.05) increased caffeine levels of the lens capsule/epithelium in the 60-, 120-, and 180-mg group. Caffeine concentrations (caffeine ng/lens capsule/epithelium) measured as difference between 1st and 2nd eye were -0.52 ± 1.16 (0-mg group, n = 7), 1.88 ± 2.02 (60-mg group, n = 8), 2.09 ± 0.67 (120-mg group, n = 9), and 3.68 ± 1.86 (180-mg group, n = 9). The increase constant of caffeine in a linear regression model was estimated as a 95% CI 0.02 ± 0.0046 (degrees of freedom; 25; r = 0.85).
Peroral intake of coffee significantly increased caffeine concentrations in the lens capsule and lens epithelial cells in a dose-dependent manner. This information is important for further investigations on preventing cataract.
确定口服摄入的咖啡因（一种广泛消费的强效膳食抗氧化剂）在前晶状体囊和晶状体上皮细胞（白内障发展的关键细胞层）中的药代动力学。
安排双侧白内障患者在双眼手术前一周内停止饮用咖啡因。在第二只眼睛手术当天，患者饮用无咖啡因饮品（0-mg 组）或含有 60、120 或 180mg 咖啡因的咖啡。在撕囊后，将包含晶状体上皮细胞的晶状体囊转移到试管中，通过气相色谱-质谱联用（GC-MS/MS）分析咖啡因浓度。
咖啡摄入显著（P < 0.05）增加了 60、120 和 180mg 组晶状体囊/上皮中的咖啡因水平。通过比较第一只眼和第二只眼测量的咖啡因浓度（咖啡因 ng/晶状体囊/上皮），0-mg 组为 -0.52 ± 1.16（n = 7），60-mg 组为 1.88 ± 2.02（n = 8），120-mg 组为 2.09 ± 0.67（n = 9），180-mg 组为 3.68 ± 1.86（n = 9）。线性回归模型中咖啡因的增长常数估计为 0.02 ± 0.0046（自由度；25；r = 0.85）。
口服摄入咖啡以剂量依赖的方式显著增加了晶状体囊和晶状体上皮细胞中的咖啡因浓度。这些信息对于进一步研究预防白内障具有重要意义。
