Vienna Institute for Research in Ocular Surgery, Department of Ophthalmology, Hanusch Hospital Vienna, Vienna, Austria.
Clinical Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
Ophthalmic Res. 2020;63(6):533-540. doi: 10.1159/000507026. Epub 2020 Mar 9.
Caffeine and its metabolites have antioxidant activity, scavenging reactive oxygen species. The aim of our study was to measure caffeine concentrations in vitreous samples after peroral caffeine intake.
This prospective study included patients scheduled for 23-G pars plana vitrectomy with membrane peeling due to epiretinal membranes. The study was performed in two parts: in the first part, patients were recruited into three different groups: group A consisted of habitual coffee drinkers who agreed to drink coffee containing 180 mg caffeine 1 h before surgery (n = 10), group B consisted of habitual coffee drinkers who were not offered coffee before surgery (n = 5), and group C consisted of non-habitual coffee drinkers, forming the control group (n = 5). In the second part (group D) patients (habitual coffee drinkers) agreed to give additional blood serum samples for measurement of caffeine concentration. Harvested samples of vitreous (groups A-D), epiretinal membranes (groups A-C), and blood serum samples (group D) were examined for concentrations of caffeine with gas chromatography-mass spectrometry.
Samples of 40 eyes of 40 patients were harvested. The concentrations of caffeine in the vitreous samples were 1,998 ± 967 ng/mL in group A and 1,108 ± 874 ng/mL in group B. In group C, caffeine concentrations were below 176 ng/mL in all vitreous samples. Both groups A and B had significantly higher concentrations of caffeine in the vitreous samples than group C (p < 0.002, p < 0.01, Mann-Whitney U test). Caffeine concentrations in epiretinal membranes were below the limits of detection. Correlation of caffeine concentrations between blood serum samples and vitreous samples in group D was high, with significantly higher caffeine concentrations in the blood serum.
Coffee consumption leads to significant caffeine levels in the vitreous compared to patients in the control group, and caffeine concentrations in the vitreous showed a high correlation to blood serum concentrations of caffeine after peroral coffee consumption.
咖啡因及其代谢物具有抗氧化活性，可清除活性氧。我们研究的目的是测量口服咖啡因后玻璃体内样本中的咖啡因浓度。
这项前瞻性研究纳入了因视网膜前膜而接受 23-G 经睫状体平坦部玻璃体切除术联合膜剥除术的患者。该研究分为两部分：在第一部分中，患者被招募到三个不同的组中：组 A 由习惯性饮用咖啡的患者组成，他们同意在手术前 1 小时饮用含有 180 毫克咖啡因的咖啡（n = 10），组 B 由未在手术前提供咖啡的习惯性饮用咖啡的患者组成（n = 5），组 C 由非习惯性饮用咖啡的患者组成，作为对照组（n = 5）。在第二部分（组 D）中，患者（习惯性饮用咖啡者）同意提供额外的血清样本以测量咖啡因浓度。用气相色谱-质谱法检测玻璃体内（组 A-D）、视网膜前膜（组 A-C）和血清样本（组 D）中咖啡因的浓度。
共采集了 40 例患者 40 只眼的样本。组 A 中玻璃体内样本的咖啡因浓度为 1998 ± 967ng/mL，组 B 中为 1108 ± 874ng/mL。在组 C 中，所有玻璃体内样本的咖啡因浓度均低于 176ng/mL。组 A 和 B 的玻璃体内样本中的咖啡因浓度均明显高于组 C（p < 0.002，p < 0.01，Mann-Whitney U 检验）。视网膜前膜中的咖啡因浓度低于检测限。在组 D 中，血清样本和玻璃体内样本之间的咖啡因浓度相关性较高，且血清中咖啡因浓度明显更高。
与对照组患者相比，咖啡消耗会导致玻璃体内的咖啡因水平显著升高，且口服咖啡后玻璃体内的咖啡因浓度与血清中的咖啡因浓度呈高度相关性。
