McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
J Tissue Eng Regen Med. 2018 Jun;12(6):1374-1388. doi: 10.1002/term.2668. Epub 2018 May 16.
The challenge of developing scaffolds to reconstruct critical-sized calvarial defects without the addition of high levels of exogenous growth factor remains relevant. Both osteogenic regenerative efficacy and suitable mechanical properties for the temporary scaffold system are of importance. In this study, a Mg alloy mesh reinforced polymer/demineralized bone matrix (DBM) hybrid scaffold was designed where the hybrid scaffold was fabricated by a concurrent electrospinning/electrospraying of poly(lactic-co-glycolic acid) (PLGA) polymer and DBM suspended in hyaluronic acid (HA). The Mg alloy mesh significantly increased the flexural strength and modulus of PLGA/DBM hybrid scaffold. In vitro results demonstrated that the Mg alloy mesh reinforced PLGA/DBM hybrid scaffold (Mg-PLGA@HA&DBM) exhibited a stronger ability to promote the proliferation of bone marrow stem cells (BMSCs) and induce BMSC osteogenic differentiation compared with control scaffolding materials lacking critical components. In vivo osteogenesis studies were performed in a rat critical-sized calvarial defect model and incorporated a variety of histological stains and immunohistochemical staining of osteocalcin. At 12 weeks, the rat model data showed that the degree of bone repair for the Mg-PLGA@HA&DBM scaffold was significantly greater than for those scaffolds lacking one or more of the principal components. Although complete defect filling was not achieved, the improved mechanical properties, promotion of BMSC proliferation and induction of BMSC osteogenic differentiation, and improved promotion of bone repair in the rat critical-sized calvarial defect model make Mg alloy mesh reinforced PLGA/DBM hybrid scaffold an attractive option for the repair of critical-sized bone defects where the addition of exogenous isolated growth factors is not employed.
开发无需添加高水平外源性生长因子即可重建临界尺寸颅骨缺损的支架仍然具有挑战性。成骨再生功效和临时支架系统的合适机械性能都很重要。在这项研究中，设计了一种镁合金网增强聚合物/脱矿骨基质（DBM）杂化支架，其中杂化支架是通过聚（乳酸-共-羟基乙酸）（PLGA）聚合物和悬浮在透明质酸（HA）中的 DBM 的共静电纺丝/静电喷雾来制备的。镁合金网显着提高了 PLGA/DBM 杂化支架的弯曲强度和模量。体外结果表明，与缺乏关键成分的对照支架材料相比，镁合金网增强的 PLGA/DBM 杂化支架（Mg-PLGA@HA&DBM）表现出更强的促进骨髓基质细胞（BMSCs）增殖和诱导 BMSC 成骨分化的能力。在大鼠临界尺寸颅骨缺损模型中进行了体内成骨研究，并进行了各种组织学染色和骨钙蛋白的免疫组织化学染色。在 12 周时，大鼠模型数据表明，Mg-PLGA@HA&DBM 支架的骨修复程度明显大于缺乏一个或多个主要成分的支架。尽管没有完全实现缺陷填充，但改善的机械性能、促进 BMSC 增殖和诱导 BMSC 成骨分化以及改善大鼠临界尺寸颅骨缺损模型中的骨修复促进作用，使镁合金网增强的 PLGA/DBM 杂化支架成为一种有吸引力的选择临界尺寸骨缺损的修复，在外源性分离生长因子的添加不被采用的情况下。
