Institute of Water Research, University of Granada, C/Ramon y Cajal, 4, 18071, Granada, Spain; Department of Civil Engineering, University of Granada, Campus of Fuentenueva, sn, 18071, Granada, Spain.
Institute of Water Research, University of Granada, C/Ramon y Cajal, 4, 18071, Granada, Spain.
Chemosphere. 2018 Aug;204:431-441. doi: 10.1016/j.chemosphere.2018.04.062. Epub 2018 Apr 13.
Three aerobic granular sequencing batch reactors were inoculated using different inocula from Finland, Spain and a mix of both in order to investigate the effect over the degradation performance and the microbial community structure. The Finnish inoculum achieved a faster granulation and a higher depollution performance within the first two month of operation. However, after 90 days of operation, similar physico-chemical values were observed. On the other hand, the Real-time PCR showed that Archaea diminished from inoculum to granular biomass, while Bacteria and Fungi numbers remained stable. All granular biomass massive parallel sequencing studies were similar regardless of the inocula from which they formed, as confirmed by singular value decomposition principal coordinates analysis, expected effect size of OTUs, and β-diversity analyses. Thermoproteaceae, Meganema and a Trischosporonaceae members were the dominant phylotypes for the three domains studied. The analysis of oligotype distribution demonstrated that a fungal oligotype was ubiquitous. The dominant OTUs of Bacteria were correlated with bioreactors performance. The results obtained determined that the microbial community structure of aerobic granular sludge was similar regardless of their inocula, showing that the granulation of biomass is related to several phylotypes. This will be of future importance for the implementation of aerobic granular sludge to full-scale systems.
采用来自芬兰、西班牙和两者混合的不同接种物接种了三个好氧颗粒序批式反应器，以研究其对降解性能和微生物群落结构的影响。芬兰接种物在前两个月的运行中实现了更快的颗粒化和更高的脱污染性能。然而，在 90 天的运行后，观察到相似的理化值。另一方面，实时 PCR 显示古菌从接种物到颗粒生物量减少，而细菌和真菌数量保持稳定。所有颗粒生物量大规模平行测序研究结果相似，这与接种物无关，这通过奇异值分解主坐标分析、OTU 的预期效应大小和β多样性分析得到证实。Thermoproteaceae、Meganema 和 Trichosporonaceae 成员是研究的三个领域的主要菌群。寡核苷酸分布分析表明，真菌寡核苷酸是普遍存在的。细菌的优势 OTUs 与生物反应器的性能相关。所得结果表明，好氧颗粒污泥的微生物群落结构与其接种物无关，表明生物量的颗粒化与几个菌群有关。这对于将好氧颗粒污泥应用于全规模系统具有重要意义。
