翻译起始因子IF3起始密码子处的AUU到AUG突变在体内消除了其自身基因（infC）的翻译自调控。
AUU-to-AUG mutation in the initiator codon of the translation initiation factor IF3 abolishes translational autocontrol of its own gene (infC) in vivo.
作者信息
Butler J S, Springer M, Grunberg-Manago M
出版信息
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4022-5. doi: 10.1073/pnas.84.12.4022.
Abstract
We previously showed that Escherichia coli translation initiation factor IF3 regulates the expression of its own gene infC at the translational level in vivo. Here we create two alterations in the infC gene and test their effects on translational autocontrol of infC expression in vivo by measuring beta-galactosidase activity expressed from infC-lacZ gene fusions under conditions of up to 4-fold derepression or 3-fold repression of infC expression. Replacement of the infC promoter with the trp promoter deletes 120 nucleotides of the infC mRNA 5' to the translation initiation site without affecting autogenous translational control. Mutation of the unusual AUU initiator codon of infC to the more common AUG initiator codon abolishes translation initiation factor IF3-dependent repression and derepression of infC expression in vivo. These results establish the AUU initiator codon of infC as an essential cis-acting element in autogenous translational control of translation initiation factor IF3 expression in vivo.
摘要
我们之前表明，大肠杆菌翻译起始因子IF3在体内翻译水平上调节其自身基因infC的表达。在此，我们对infC基因进行了两处改变，并通过在infC表达高达4倍去阻遏或3倍阻遏的条件下，测量由infC-lacZ基因融合体表达的β-半乳糖苷酶活性，来测试它们对体内infC表达的翻译自调控的影响。用trp启动子替换infC启动子会删除infC mRNA翻译起始位点上游5'端的120个核苷酸，而不影响自身翻译调控。将infC不寻常的AUU起始密码子突变为更常见的AUG起始密码子，会消除体内翻译起始因子IF3对infC表达的阻遏和去阻遏作用。这些结果确立了infC的AUU起始密码子是体内翻译起始因子IF3表达的自身翻译调控中一个必不可少的顺式作用元件。
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本文引用的文献
1
Circular dichroism study of Escherichia coli initiation factor 3 binding to nucleic acids.大肠杆菌起始因子3与核酸结合的圆二色性研究。
Biochemistry. 1980 Sep 16;19(19):4486-92. doi: 10.1021/bi00560a016.
2
Escherichia coli translational initiation factor IF3: a unique case of translational regulation.大肠杆菌翻译起始因子IF3：翻译调控的一个独特案例。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7061-5. doi: 10.1073/pnas.81.22.7061.
3
Structural and transcriptional evidence for related thrS and infC expression.thrS和infC相关表达的结构和转录证据。
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6152-6. doi: 10.1073/pnas.80.20.6152.
4
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.原核生物、真核生物和细胞器中蛋白质合成起始的比较。
Microbiol Rev. 1983 Mar;47(1):1-45. doi: 10.1128/mr.47.1.1-45.1983.
5
Nuclease mapping of the secondary structure of the 49-nucleotide 3' terminal cloacin fragment of Escherichia coli 16s RNA and its interactions with initiation factor 3.大肠杆菌16S RNA 49个核苷酸的3'末端杀鱼菌素片段二级结构的核酸酶图谱分析及其与起始因子3的相互作用。
Nucleic Acids Res. 1983 Apr 11;11(7):2035-52. doi: 10.1093/nar/11.7.2035.
6
Initiation factor and ribosome levels are coordinately controlled in Escherichia coli growing at different rates.在以不同速率生长的大肠杆菌中，起始因子和核糖体水平受到协同控制。
J Biol Chem. 1983 Feb 10;258(3):1954-9.
7
Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.包含大肠杆菌起始因子IF3结构基因的1.26千碱基DNA片段的序列：存在AUU起始密码子。
EMBO J. 1982;1(3):311-5. doi: 10.1002/j.1460-2075.1982.tb01166.x.
8
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.利用单链噬菌体克隆辅助快速DNA测序。
J Mol Biol. 1980 Oct 25;143(2):161-78. doi: 10.1016/0022-2836(80)90196-5.
9
Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.从一组与翻译相关的基因中对大肠杆菌起始因子IF3的结构基因进行物理定位和克隆。
Gene. 1980 Oct;11(1-2):33-42. doi: 10.1016/0378-1119(80)90084-0.
10
Regulation of the synthesis of ribosomes and ribosomal components.核糖体及核糖体组分合成的调控。
Annu Rev Biochem. 1984;53:75-117. doi: 10.1146/annurev.bi.53.070184.000451.
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