基于 DNA 模板银纳米簇的 NdeI 内切酶活性无标记检测
A Label-Free Detection of NdeI Endonuclease Activity by Using DNA-Templated Silver Nanoclusters.
机构信息
Department of Nano Chemistry, Gachon University, Seongnam-si, Gyeonggi-do 13120, South Korea.
出版信息
J Nanosci Nanotechnol. 2018 Sep 1;18(9):6339-6342. doi: 10.1166/jnn.2018.15657.
Restriction endonucleases play an important role in genetic recombination, molecular cloning, clinical diagnosis, and pharmacological application in drug studies. In the present study, DNA-templated Ag+ nanoclusters (DNA-AgNCs) were used to detect NdeI endonuclease activity due to their facile synthesis, outstanding optical properties, and good biocompatibility. This study monitored fluorescence intensity of DNA-AgNCs to detect NdeI endonuclease activity and its inhibition in the presence of 5-fluorouracil under optimal conditions.
限制内切酶在遗传重组、分子克隆、临床诊断以及药物研究中的药理学应用方面发挥着重要作用。在本研究中，由于其易于合成、出色的光学性质和良好的生物相容性，使用 DNA 模板化 Ag+纳米团簇（DNA-AgNCs）来检测 NdeI 内切酶活性。本研究在最佳条件下，通过监测 DNA-AgNCs 的荧光强度来检测 NdeI 内切酶活性及其在 5-氟尿嘧啶存在下的抑制作用。
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