Vermillion Katie L, Bacher Rhonda, Tannenbaum Alex P, Swanson Scott, Jiang Peng, Chu Li-Fang, Stewart Ron, Thomson James A, Vereide David T
Morgridge Institute for Research, Madison, WI 53715, USA.
Department of Biostatistics, University of Florida, Gainesville, FL 32611, USA.
Dev Biol. 2018 Jul 1;439(1):30-41. doi: 10.1016/j.ydbio.2018.04.007. Epub 2018 Apr 17.
During vertebrate development, progenitor cells give rise to tissues and organs through a complex choreography that commences at gastrulation. A hallmark event of gastrulation is the formation of the primitive streak, a linear assembly of cells along the anterior-posterior (AP) axis of the developing organism. To examine the primitive streak at a single-cell resolution, we measured the transcriptomes of individual chick cells from the streak or the surrounding tissue (the rest of the area pellucida) in Hamburger-Hamilton stage 4 embryos. The single-cell transcriptomes were then ordered by the statistical method Wave-Crest to deduce both the relative position along the AP axis and the prospective lineage of single cells. The ordered transcriptomes reveal intricate patterns of gene expression along the primitive streak.
在脊椎动物发育过程中，祖细胞通过始于原肠胚形成的复杂编排产生组织和器官。原肠胚形成的一个标志性事件是原条的形成，原条是发育中生物体沿前后（AP）轴的细胞线性集合。为了以单细胞分辨率研究原条，我们测量了汉堡-汉密尔顿4期胚胎中原条或周围组织（透明区的其余部分）的单个鸡细胞的转录组。然后通过统计方法Wave-Crest对单细胞转录组进行排序，以推断单个细胞沿AP轴的相对位置和预期谱系。排序后的转录组揭示了沿原条的复杂基因表达模式。
