Yoon Yeonsoo, Huang Tingting, Tortelote Giovane G, Wakamiya Maki, Hadjantonakis Anna-Katerina, Behringer Richard R, Rivera-Pérez Jaime A
Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555-0621, USA.
Dev Biol. 2015 Jul 1;403(1):80-8. doi: 10.1016/j.ydbio.2015.04.008. Epub 2015 Apr 20.
The establishment of the head to tail axis at early stages of development is a fundamental aspect of vertebrate embryogenesis. In mice, experimental embryology, genetics and expression studies have suggested that the visceral endoderm, an extra-embryonic tissue, plays an important role in anteroposterior axial development. Here we show that absence of Wnt3 in the posterior visceral endoderm leads to delayed formation of the primitive streak and that interplay between anterior and posterior visceral endoderm restricts the position of the primitive streak. Embryos lacking Wnt3 in the visceral endoderm, however, appear normal by E9.5. Our results suggest a model for axial development in which multiple signals are required for anteroposterior axial development in mammals.
在发育早期建立从头到尾的轴是脊椎动物胚胎发生的一个基本方面。在小鼠中，实验胚胎学、遗传学和表达研究表明，脏内胚层（一种胚外组织）在前后轴发育中起重要作用。在这里，我们表明脏内胚层后部缺乏Wnt3会导致原条形成延迟，并且前后脏内胚层之间的相互作用限制了原条的位置。然而，在内脏内胚层中缺乏Wnt3的胚胎在E9.5时看起来是正常的。我们的结果提出了一个轴向发育模型，其中哺乳动物的前后轴发育需要多种信号。
