Grupo de Investigación en Sustancias Bioactivas, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia.
Departamento de Ciencias Básicas, Universidad Católica Luis Amigó, SISCO, Cross 51A No. 67B-90. Medellín, Colombia.
Life Sci. 2018 Jun 15;203:54-65. doi: 10.1016/j.lfs.2018.04.026. Epub 2018 Apr 18.
Determine a relationship between the neuroprotective activity and the antioxidant capacity of the Amaryllidaceae alkaloids in a model of Glu excitotoxicity in rat cortical neurons.
Was evaluated several alkaloidal fractions isolated from Amaryllidaceae species, a family known to contain neuroprotective alkaloids, in a model of Glu excitotoxicity in rat cortical neurons. In addition, several mechanisms of antioxidant activity were used, and a theoretical study of the antioxidants was performed.
The results of this study suggest that a possible neuroprotective mode of action of the alkaloidal fractions of Eucharis bonplandii (Kunth) Traub bulbs, Eucharis caucana Meerow bulbs, and Clivia miniata Regel leaves, is through their antioxidant activity and ability to stabilize free radicals generated from an excitotoxic process mediated by Glu. The chemical structure characterization and antioxidant activity of the fractions suggest that the phenol and enol groups in the structures of the alkaloids are critical for the stabilization of ROS and RNS. Additionally, the pair of free electrons on the N is spatially close to a hydroxyl group, which benefits the cleavage of this group and, consequently, the stabilization of the generated O.
The versatility of the structures of the studied Amaryllidaceae alkaloids suggests that they have potential as neuroprotective agents against an oxidative stimulus.
在大鼠皮质神经元谷氨酸兴奋性毒性模型中，确定石蒜科生物碱的神经保护活性与抗氧化能力之间的关系。
评估了几种从石蒜科物种中分离出的生物碱类化合物，该科含有具有神经保护作用的生物碱，在大鼠皮质神经元谷氨酸兴奋性毒性模型中进行了评估。此外，还使用了几种抗氧化活性机制，并对抗氧化剂进行了理论研究。
这项研究的结果表明，Eucharis bonplandii（Kunth）Traub 鳞茎、Eucharis caucana Meerow 鳞茎和 Clivia miniata Regel 叶的生物碱类化合物的可能神经保护作用机制是通过其抗氧化活性和稳定由 Glu 介导的兴奋毒性过程中产生的自由基的能力。该类化合物的化学结构特征和抗氧化活性表明，其结构中的酚和烯醇基团对于稳定 ROS 和 RNS 至关重要。此外，N 上的一对自由电子与羟基在空间上接近，这有利于该基团的断裂，从而稳定生成的 O。
所研究的石蒜科生物碱的结构的多功能性表明，它们具有作为针对氧化应激的神经保护剂的潜力。
