Institute of Pesticide and Environmental Toxicology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China.
Institute of Pesticide and Environmental Toxicology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China.
Ecotoxicol Environ Saf. 2018 Aug 30;158:145-153. doi: 10.1016/j.ecoenv.2018.04.026. Epub 2018 Apr 24.
The biodegradability and ecological safety assessment of the previously isolated DDT-degrading bacterial strain Stenotrophomonas sp. DDT-1 were investigated in the DDT-contaminated soil under laboratory and field conditions. Under laboratory conditions, the degradation rates of fresh p,p'-DDT in soil were enhanced by 2.0-3.0-fold with the introduction of the strain DDT-1 compared to those of the control treatments. A similar enhancement in the dissipation of DDTs (p,p'-DDT, p,p'-DDE, p,p'-DDD, and o,p'-DDT) in the aged DDT-contaminated field plot soils resulted from the inoculation with this strain. Meanwhile, the degradation rates of DDTs increased by 2.9-5.5- and 2.8-7.6-fold in the inoculated greenhouse and open field soils, respectively, after field demonstration application of strain DDT-1 preparation. Moreover, no significant differences in the soil enzyme activity, microbial functional diversity, and bacterial community structure were observed between the inoculated and un-inoculated field soils, but several soil microbial genera exhibited some fluctuations in abundance. It is concluded that strain DDT-1 could accelerate the removal of DDTs residues in field soils, and furthermore, its inoculation was ecologically safe.
在实验室和田间条件下，研究了先前分离的 DDT 降解细菌 Stenotrophomonas sp. DDT-1 在 DDT 污染土壤中的生物降解性和生态安全性评估。在实验室条件下，与对照处理相比，引入菌株 DDT-1 可将新鲜 p,p'-DDT 在土壤中的降解率提高 2.0-3.0 倍。在接种该菌株后，老化的 DDT 污染田间土壤中 DDTs（p,p'-DDT、p,p'-DDE、p,p'-DDD 和 o,p'-DDT）的消解也得到了类似的增强。同时，在田间示范应用 DDT-1 制剂后，温室和露天土壤中 DDTs 的降解率分别提高了 2.9-5.5-和 2.8-7.6 倍。此外，在接种和未接种田间土壤之间，土壤酶活性、微生物功能多样性和细菌群落结构没有观察到显著差异，但一些土壤微生物属的丰度出现了一些波动。结论是，菌株 DDT-1 可以加速田间土壤中 DDT 残留的去除，并且其接种是生态安全的。
