Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya, 60111, Indonesia.
Department of Forest and Environmental Science, Faculty of Agriculture, University of Miyazaki, 1-1, Gakuen-kibanadai-nishi, Miyazaki, 889-2192, Japan.
Curr Microbiol. 2021 Feb;78(2):668-678. doi: 10.1007/s00284-020-02305-8. Epub 2021 Jan 5.
The residue of organochlorine pesticides (OCPs) has been a major pollution problem in our environment. Dichlorodiphenyltrichloroethane (DDT) is one of the most common persistent OCPs that continue to pose a serious risk to human health and the environment. Some treatment methods have been developed to reduce and minimize the adverse impacts of the use of DDT, including biodegradation with brown-rot fungi (BRF). However, DDT degradation using BRF has still low degradation rate and needs a long incubation time. Therefore, the ability of BRF need to be enhanced to degrade DDT. Interaction and effect of bacteria addition on biodegradation of DDT by brown-rot fungus Daedalea dickinsii were investigated. The interaction assay between D. dickinsii with bacteria addition showed that the addition of bacterium Pseudomonas aeruginosa did not provide resistance to the growth of D. dickinsii. Meanwhile, bacterium Bacillus subtilis addition has an inhibitory effect on the growth of D. dickinsii. The addition of 10 ml (1 ml = 1.05 × 10 CFU/ml bacteria cell) of P. aeruginosa and B. subtilis was able to improve DDT biodegradation by D. dickinsii from 53.61% to 96.70% and 67.60%, respectively. The highest biodegradation capability of DDT was obtained through addition of 10 ml of P. aeruginosa into the D. dickinsii culture in which the mixed cultures produce final metabolites of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD) and 1-chloro-2,2-bis(4-chlorophenyl)ethylene (DDMU). This study indicated that the addition of P. aeruginosa can be used for optimization of DDT biodegradation by D. dickinsii.
有机氯农药（OCPs）残留一直是我们环境中的一个主要污染问题。滴滴涕（DDT）是最常见的持久性 OCPs 之一，它继续对人类健康和环境构成严重威胁。已经开发了一些处理方法来减少和最小化使用滴滴涕的负面影响，包括用棕腐菌（BRF）进行生物降解。然而，用 BRF 降解 DDT 的降解率仍然很低，需要很长的培养时间。因此，需要增强 BRF 的能力来降解 DDT。研究了添加细菌对棕腐菌 Daedalea dickinsii 降解滴滴涕的相互作用和影响。D. dickinsii 与添加细菌的相互作用试验表明，添加铜绿假单胞菌（Pseudomonas aeruginosa）并没有为 D. dickinsii 的生长提供抗性。同时，添加枯草芽孢杆菌（Bacillus subtilis）对 D. dickinsii 的生长有抑制作用。添加 10ml（1ml=1.05×10CFU/ml 细菌细胞）的铜绿假单胞菌和枯草芽孢杆菌能够分别将 D. dickinsii 对 DDT 的生物降解率从 53.61%提高到 96.70%和 67.60%。通过向 D. dickinsii 培养物中添加 10ml 铜绿假单胞菌，可获得最高的 DDT 生物降解能力，混合培养物产生的最终代谢物为 1,1-二氯-2,2-双（4-氯苯基）乙烷（DDD）和 1-氯-2,2-双（4-氯苯基）乙烯（DDMU）。本研究表明，添加铜绿假单胞菌可用于优化 D. dickinsii 对 DDT 的生物降解。
