Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
Department of Pathology, Oncology Institute, Istanbul University, Istanbul, Turkey.
Pharmacol Rep. 2018 Jun;70(3):584-590. doi: 10.1016/j.pharep.2017.12.005. Epub 2017 Dec 19.
Methylglyoxal (MG) is a highly reactive dicarbonyl compound. It is produced by processes like glycolysis, glucose autooxidation, lipid peroxidation, and protein glycation. It is a major precursor of advanced glycation end products (AGE). It also exacerbates oxidative stress in the organism. Although there are some in vitro studies investigating the effect of resveratrol (RES) as an antioxidant and antiglycating agent on MG-induced toxicity, in vivo effect of RES is unknown. Therefore, we aimed to investigate the efficiency of RES in chronic MG-treated rats.
Rats were given incrementally increased doses (100-300 mg/kg) of MG in drinking water for ten weeks. RES (10 mg/kg ip) was administered together with MG. Reactive oxygen species (ROS) formation, thiobarbituric reactive substances (TBARS), protein carbonyl (PC), advanced oxidation protein products (AOPP) and AGE levels as well as ferric reducing antioxidant power (FRAP) values were determined in plasma and liver.
Significant increases in plasma TBARS, PC, AOPP and AGE and fructosamine levels were detected in MG-treated rats. However, plasma ROS and FRAP levels remained unchanged. Hepatic ROS, TBARS, PC and AOPP, but not AGE and FRAP levels were also increased in MG-treated rats. RES treatment diminished high levels of plasma PC, AOPP and AGE levels in MG-treated rats. Additionally, significant decreases in hepatic ROS, TBARS, PC and AOPP levels together with histopatological amelioration were detected due to RES treatment in MG-treated rats.
Our results indicate that RES may be considered as a protective agent against glycoxidative stress generated by in vivo MG treatment.
甲基乙二醛（MG）是一种高度反应性的二羰基化合物。它是通过糖酵解、葡萄糖自动氧化、脂质过氧化和蛋白质糖化等过程产生的。它是高级糖基化终产物（AGE）的主要前体。它还会加剧机体的氧化应激。虽然有一些体外研究调查了白藜芦醇（RES）作为抗氧化剂和抗糖化剂对 MG 诱导的毒性的影响，但 RES 的体内效应尚不清楚。因此，我们旨在研究 RES 在慢性 MG 处理大鼠中的功效。
大鼠在饮用水中逐渐增加剂量（100-300mg/kg）的 MG 持续十周。同时给予 RES（10mg/kg ip）。在血浆和肝脏中测定活性氧（ROS）形成、硫代巴比妥酸反应物质（TBARS）、蛋白质羰基（PC）、高级氧化蛋白产物（AOPP）和 AGE 水平以及铁还原抗氧化能力（FRAP）值。
MG 处理大鼠的血浆 TBARS、PC、AOPP 和 AGE 以及果糖胺水平显著升高。然而，血浆 ROS 和 FRAP 水平保持不变。MG 处理大鼠的肝 ROS、TBARS、PC 和 AOPP 也增加，但 AGE 和 FRAP 水平没有增加。RES 治疗可降低 MG 处理大鼠血浆中高 PC、AOPP 和 AGE 水平。此外，RES 治疗还可降低 MG 处理大鼠肝 ROS、TBARS、PC 和 AOPP 水平，同时改善组织病理学。
我们的结果表明，RES 可被视为一种针对体内 MG 处理产生的糖基化应激的保护剂。
