Bingul Ilknur, Olgac Vakur, Bekpinar Seldag, Uysal Mujdat
Department of Medical Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Department of Pathology, Institute of Oncology, Istanbul University, Istanbul, Turkey.
Arch Physiol Biochem. 2021 Dec;127(6):551-556. doi: 10.1080/13813455.2019.1659826. Epub 2019 Sep 2.
The immunosuppressive agent cyclosporine A (CsA) has hepatotoxic potential. Increased reactive oxygen species (ROS) formation is among the causes leading to hepatotoxicity. This study aimed to investigate the effect of resveratrol (RES) on CsA-induced oxidative stress and hepatotoxicity in rats. Rats were treated with RES (10 mg/kg/day; i.p.) for 14 days. CsA (25 mg/kg/day; s.c.) was given during the last seven days together with RES. Serum alanine aminotransferase and aspartate aminotransferase activities together with hepatic histopathological examinations were performed. ROS, thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPPs), ferric reducing antioxidant power, and glutathione levels as well as superoxide dismutase, and glutathione peroxidase activities were measured in the liver tissue. RES ameliorated histopathological changes and decreased hepatic ROS, TBARS, and AOPP levels significantly. However, antioxidant parameters did not change in CsA-treated rats. Our results indicate that RES treatment may be effective in decreasing CsA-induced oxidative stress and hepatotoxicity.
免疫抑制剂环孢素A（CsA）具有肝毒性潜力。活性氧（ROS）生成增加是导致肝毒性的原因之一。本研究旨在探讨白藜芦醇（RES）对CsA诱导的大鼠氧化应激和肝毒性的影响。大鼠接受RES（10毫克/千克/天；腹腔注射）治疗14天。在最后7天，CsA（25毫克/千克/天；皮下注射）与RES一起给药。进行了血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性检测以及肝脏组织病理学检查。在肝脏组织中测量了ROS、硫代巴比妥酸反应性物质（TBARS）、晚期氧化蛋白产物（AOPPs）、铁还原抗氧化能力、谷胱甘肽水平以及超氧化物歧化酶和谷胱甘肽过氧化物酶活性。RES改善了组织病理学变化，并显著降低了肝脏ROS、TBARS和AOPP水平。然而，接受CsA治疗的大鼠的抗氧化参数没有变化。我们的结果表明，RES治疗可能有效降低CsA诱导的氧化应激和肝毒性。
