Hashemzaei Mahmoud, Tabrizian Kaveh, Alizadeh Zeinab, Pasandideh Sedigheh, Rezaee Ramin, Mamoulakis Charalampos, Tsatsakis Aristidis, Skaperda Zoi, Kouretas Demetrios, Shahraki Jafar
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
Toxicology and Addiction Research Center, Zabol University of Medical Sciences, Zabol, Iran.
Toxicol Rep. 2020 Nov 23;7:1571-1577. doi: 10.1016/j.toxrep.2020.11.008. eCollection 2020.
Glyoxal (GO), a by-product of glucose auto-oxidation, is involved in the glycation of proteins/ lipids and formation of advanced glycation (AGE) and lipoxidation (ALE) end products. AGE/ALE were shown to contribute to diabetic complications development/progression such as nephropathy. Diabetic nephropathy progression has an oxidative nature. Given the antioxidant effects of polyphenols, potential protective effects of resveratrol, curcumin and gallic acid, in rat renal cells treated with GO, were evaluated in the present work. According to our results, incubation of GO with the cells reduced their viability and led to membrane lysis, reactive oxygen species (ROS) formation, lipid peroxidation, mitochondrial membrane potential collapse, and lysosomal membrane leakage. These findings were prevented by pre-treatment with resveratrol, curcumin and gallic acid. Mitochondrial and lysosomal toxic interactions appear to worsen oxidative stress/cytotoxicity produced by GO. Resveratrol, curcumin and gallic acid inhibited ROS formation and attenuated GO-induced renal cell death.
乙二醛（GO）是葡萄糖自氧化的副产物，参与蛋白质/脂质的糖基化以及晚期糖基化终产物（AGE）和脂氧化终产物（ALE）的形成。研究表明，AGE/ALE会促使糖尿病并发症的发展/进展，如肾病。糖尿病肾病的进展具有氧化性质。鉴于多酚类物质的抗氧化作用，本研究评估了白藜芦醇、姜黄素和没食子酸在经GO处理的大鼠肾细胞中的潜在保护作用。根据我们的结果，将细胞与GO共同孵育会降低其活力，并导致细胞膜裂解、活性氧（ROS）形成、脂质过氧化、线粒体膜电位崩溃和溶酶体膜渗漏。白藜芦醇、姜黄素和没食子酸预处理可防止这些结果的出现。线粒体和溶酶体的毒性相互作用似乎会加剧GO所产生的氧化应激/细胞毒性。白藜芦醇、姜黄素和没食子酸可抑制ROS形成，并减轻GO诱导的肾细胞死亡。
