Lorenzo de Arriba M, Lopez-Serrano S, Galofre-Mila N, Aragon V
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Vet J. 2018 Apr;234:1-6. doi: 10.1016/j.tvjl.2018.01.004. Epub 2018 Feb 6.
The aim of this study was to characterise bacteria in the genus Bergeyella isolated from the nasal passages of healthy piglets. Nasal swabs from 3 to 4 week-old piglets from eight commercial domestic pig farms and one wild boar farm were cultured under aerobic conditions. Twenty-nine Bergeyella spp. isolates were identified by partial 16S rRNA gene sequencing and 11 genotypes were discriminated by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Bergeyella zoohelcum and Bergeyella porcorum were identified within the 11 genotypes. Bergeyella spp. isolates exhibited resistance to serum complement and phagocytosis, poor capacity to form biofilms and were able to adhere to epithelial cells. Maneval staining was consistent with the presence of a capsule. Multiple drug resistance (resistance to three or more classes of antimicrobial agents) was present in 9/11 genotypes, including one genotype isolated from wild boar with no history of antimicrobial use. In conclusion, Bergeyella spp. isolates from the nasal cavities of piglets showed some in vitro features indicative of a potential for virulence. Further studies are necessary to identify the role of Bergeyella spp. in disease and within the nasal microbiota of pigs.
本研究的目的是对从健康仔猪鼻腔分离出的伯格氏菌属细菌进行特征描述。从八个商业家养猪场和一个野猪场采集3至4周龄仔猪的鼻拭子，在需氧条件下进行培养。通过部分16S rRNA基因测序鉴定出29株伯格氏菌属菌株，并通过肠杆菌重复基因间共识（ERIC）-PCR区分出11种基因型。在这11种基因型中鉴定出了人兽伯格氏菌和猪伯格氏菌。伯格氏菌属菌株表现出对血清补体和吞噬作用的抗性、形成生物膜的能力较差，并且能够粘附于上皮细胞。马尼瓦尔染色与荚膜的存在一致。9/11种基因型存在多重耐药性（对三类或更多类抗菌药物耐药），其中包括从无抗菌药物使用史的野猪分离出的一种基因型。总之，从仔猪鼻腔分离出的伯格氏菌属菌株显示出一些体外特征，表明其具有潜在的毒力。有必要进一步研究以确定伯格氏菌属在猪疾病和鼻腔微生物群中的作用。
