Pessoa L-S, Pereira-da Silva F-R, Alves E-H-P, França L-F-C, di Lenardo D, Carvalho J-S, Martins V-B-D, Sousa F-B-M, Drumond K-O, Medeiros J-V-R, de Oliveira J-S, Vasconcelos D-F-P
Universidade Federal do Piauí - UFPI, Campus Ministro Reis Veloso, Colegiado de Biomedicina, Av. São Sebastião, 2819, Reis Veloso, Parnaiba - PI, Brazil 64204-035,
Med Oral Patol Oral Cir Bucal. 2018 May 1;23(3):e269-e276. doi: 10.4317/medoral.22204.
Periodontitis is a chronic disease that due to an intense inflammatory response triggers systemic changes such as hepatic alterations. This study aimed to compare hepatic damage in rats that received experimental periodontitis at one or two periodontal sites with ligatures.
Eighteen rats were separated into three groups: control, without ligature; periodontitis 1, with one ligature; and periodontitis 2, with two ligatures. The following parameters were assessed: gingival bleeding index, probing pocket depth, tooth mobility, alveolar bone loss, malondialdehyde (MDA) and myeloperoxidase (MPO) activity in periodontal tissue; histopathological evaluation of hepatic tissue (steatosis score); glutathione levels (GSH), MDA, MPO, cholesterol and triglycerides in the liver; and serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST).
Periodontal evaluation data showed that the periodontitis model worked well. The groups with periodontitis did not differ significantly in relation to MPO activity and MDA levels in the gingival samples, but they were significantly different when compared with the control group. Steatosis was observed in the histological analysis of the groups with periodontitis, but between the periodontitis groups, two ligatures did not cause increase in steatosis score. The levels of GSH, MDA, total cholesterol and triglycerides in the hepatic tissue were not altered between groups with periodontitis, but they showed significant differences in comparison with the control group. The activity of MPO in hepatic tissue and serum levels of AST and ALT did not present significant difference among the three groups.
In conclusion, our results demonstrated that one or two ligatures inducing periodontitis were both sufficient to cause fatty liver. Steatosis caused by two ligatures did not present larger extension and severity than steatosis caused by one ligature.
牙周炎是一种慢性疾病，由于强烈的炎症反应会引发全身变化，如肝脏改变。本研究旨在比较在一个或两个牙周部位通过结扎诱导实验性牙周炎的大鼠的肝脏损伤情况。
将18只大鼠分为三组：对照组，不进行结扎；牙周炎1组，进行一处结扎；牙周炎2组，进行两处结扎。评估以下参数：牙龈出血指数、探诊深度、牙齿松动度、牙槽骨吸收、牙周组织中的丙二醛（MDA）和髓过氧化物酶（MPO）活性；肝组织的组织病理学评估（脂肪变性评分）；肝脏中的谷胱甘肽水平（GSH）、MDA、MPO、胆固醇和甘油三酯；以及血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平。
牙周评估数据表明牙周炎模型效果良好。牙周炎组在牙龈样本中的MPO活性和MDA水平方面差异不显著，但与对照组相比有显著差异。在牙周炎组的组织学分析中观察到脂肪变性，但在牙周炎组之间，两处结扎并未导致脂肪变性评分增加。牙周炎组之间肝组织中的GSH、MDA、总胆固醇和甘油三酯水平未发生改变，但与对照组相比有显著差异。肝组织中的MPO活性以及血清AST和ALT水平在三组之间无显著差异。
总之，我们的结果表明，一处或两处结扎诱导牙周炎均足以导致脂肪肝。两处结扎引起的脂肪变性在范围和严重程度上并不比一处结扎引起的脂肪变性更大。
