Laboratório de Bioquímica de Plantas Laticíferas (LABPL), Universidade Federal do Delta do Parnaíba, Parnaíba, Brazil.
Laboratório de Análise e Processamento Histológico (LAPHis), Universidade Federal do Delta do Parnaíba, Parnaíba, Brazil.
Oral Dis. 2022 Apr;28(3):786-795. doi: 10.1111/odi.13803. Epub 2021 Feb 23.
Previous studies have shown that latex proteins from Plumeria pudica (LPPp) have anti-inflammatory and antioxidant activity. Therefore, the aim of this study was to evaluate the effects in rats of LPPp on ligature-induced periodontitis, an inflammatory disease.
The animals were divided into groups: saline (animals without induction of periodontitis), periodontitis (induced periodontitis and untreated) and LPPp (induced periodontitis and treated with 40 mg/kg). The following parameters were evaluated after 20 consecutive days of treatment: gingival bleeding index (GBI), probing pocket depth (PPD), alveolar bone height (ABH) and gingival myeloperoxidase (MPO) activity. In the hepatic tissue, malondialdehyde (MDA), glutathione (GSH) and histopathological alterations were evaluated. Blood levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured.
Significant reduction in GBI, PPD and gingival MPO activity and ABH was seen in animals treated with LPPp compared with periodontitis. Values of GSH, MDA, ALT and histopathological evaluation were preserved in animals treated with LPPp.
Treatment with LPPp improved clinical aspects of periodontitis, reduced the blood and hepatic alterations and prevented alveolar bone loss. Data suggest that LPPp have potential for treatment of periodontitis.
先前的研究表明，来自黄蝉（Plumeria pudica）的乳胶蛋白（LPPp）具有抗炎和抗氧化活性。因此，本研究旨在评估 LPPp 对结扎诱导的牙周炎（一种炎症性疾病）在大鼠中的作用。
动物分为以下几组：生理盐水组（无牙周炎诱导的动物）、牙周炎组（诱导牙周炎且未治疗）和 LPPp 组（诱导牙周炎并用 40mg/kg 治疗）。经过 20 天的连续治疗后，评估以下参数：牙龈出血指数（GBI）、探诊袋深度（PPD）、牙槽骨高度（ABH）和牙龈髓过氧化物酶（MPO）活性。在肝组织中，评估丙二醛（MDA）、谷胱甘肽（GSH）和组织病理学改变。测量血液中丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的水平。
与牙周炎组相比，LPPp 治疗的大鼠 GBI、PPD 和牙龈 MPO 活性以及 ABH 显著降低。LPPp 治疗的大鼠 GSH、MDA、ALT 和组织病理学评估值得以维持。
LPPp 治疗改善了牙周炎的临床症状，减少了血液和肝脏的改变，并防止了牙槽骨丢失。数据表明，LPPp 具有治疗牙周炎的潜力。
