Center of Innovative and Applied Bioprocessing, Sector-81 (Knowledge City), S.A.S. Nagar, Mohali, Punjab, 140 306, India.
Bioprocess Biosyst Eng. 2018 Aug;41(8):1121-1131. doi: 10.1007/s00449-018-1941-0. Epub 2018 Apr 21.
The study investigated an integrated bioprocessing of raw and by-products from sugarcane and dairy industries for production of non-digestible prebiotic and functional ingredients. The low-priced feedstock, whey, molasses, table sugar, jaggery, etc., were subjected to transglucosylation reactions catalyzed by dextransucrase from Leuconostoc mesenteroides MTCC 10508. HPLC analysis approximated production of about 11-14 g L trisaccharide i.e. 2-α-D-glucopyranosyl-lactose (4-galactosyl-kojibiose) from the feedstock prepared from table sugar, jaggery, cane molasses and liquid whey, containing about 30 g L sucrose and lactose each. The trisaccharide was hydrolysed into the prebiotic disaccharide, kojibiose, by employing recombinant β-galactosidase from Escherichia coli. The enzyme β-galactosidase achieved about 90% conversion of 2-α-D-glucopyranosyl-lactose into kojibiose. The D-fructose generated by catalytic reactions of dextransucrase was targeted for catalytic transformation into rare sugar, D-allulose (or D-psicose), by treating the samples with Smt3-D-psicose 3-epimerase. The catalytic reactions resulted in the conversion of ~ 25% D-fructose to D-allulose. These bioactive compounds are known to exert a plethora of benefits to human health, and therefore, are preferred ingredients for making functional foods.
该研究调查了甘蔗和乳制品工业的原料和副产品的综合生物加工，以生产不可消化的益生元和功能性成分。利用来自 Leuconostoc mesenteroides MTCC 10508 的葡聚糖蔗糖酶进行转葡糖苷反应，对价格低廉的原料乳清、糖蜜、白砂糖、糖蜜等进行了处理。HPLC 分析表明，从由白砂糖、糖蜜、甘蔗糖蜜和液态乳清制备的原料中可生产约 11-14 g/L 的三糖，即 2-α-D-吡喃葡萄糖基乳糖（4-半乳糖基-低聚异麦芽糖）。每升含有约 30 g/L 的蔗糖和乳糖。利用来自大肠杆菌的重组β-半乳糖苷酶将三糖水解成益生元二糖低聚异麦芽糖。β-半乳糖苷酶将 2-α-D-吡喃葡萄糖基乳糖转化为低聚异麦芽糖的转化率约为 90%。葡聚糖蔗糖酶的催化反应生成的 D-果糖通过用 Smt3-D-阿洛酮糖 3-差向异构酶处理样品，靶向催化转化为稀有糖 D-阿洛酮糖（或 D-塔格糖）。催化反应将约 25%的 D-果糖转化为 D-阿洛酮糖。这些生物活性化合物已知对人类健康有多种益处，因此是制造功能性食品的首选成分。
