Bampali Evangelia, Graikou Konstantia, Aligiannis Nektarios, Chinou Ioanna
Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis-Zografou, 15771 Athens, Greece.
Evid Based Complement Alternat Med. 2018 Feb 26;2018:6802753. doi: 10.1155/2018/6802753. eCollection 2018.
The chemical composition, as well as the total phenolic content (TPC) and the potential antioxidant and antimicrobial activity, of three Kainari-herbal tea samples from different areas of Lesvos Island (Greece) was evaluated. The rich aroma of the mixtures was studied through GC-MS, as well as through Headspace Solid-Phase Microextraction (HS-SPME)/GC-MS analyses. Cinnamon, clove, nutmeg, pepper, and ginger were identified as main ingredients, while, throughout the chemical analysis of the volatiles of one selected sample, several secondary metabolites have been isolated and identified on the basis of GC-MS as well as spectral evidence as eugenol, cinnamic aldehyde and myristicin, cinnamyl alcohol, alpha-terpinyl acetate, and -caryophyllene. Furthermore, two food dyes, azorubine and amaranth, were also isolated and identified from the infusions. The total phenolic content was estimated and the free radical scavenging activity was determined by DPPH and ABTS assays and the antimicrobial activity of the extracts was tested showing a very interesting profile against all the assayed microorganisms. Due to its very pleasant aroma and taste properties as well as to its bioactivities, Kainari-herbal tea could be further proposed as functional beverage.
对来自希腊莱斯博斯岛不同地区的三个卡伊纳里草本茶样品的化学成分、总酚含量(TPC)以及潜在的抗氧化和抗菌活性进行了评估。通过气相色谱 - 质谱联用(GC-MS)以及顶空固相微萃取(HS-SPME)/GC-MS分析研究了混合物浓郁的香气。肉桂、丁香、肉豆蔻、胡椒和姜被鉴定为主要成分,同时,在对一个选定样品的挥发物进行化学分析过程中,基于GC-MS以及光谱证据,分离并鉴定出了几种次生代谢产物,如丁香酚、肉桂醛、肉豆蔻醚、肉桂醇、乙酸α - 萜品酯和β - 石竹烯。此外,还从浸出液中分离并鉴定出了两种食用色素,偶氮玉红和苋菜红。测定了总酚含量,并通过DPPH和ABTS试验测定了自由基清除活性,测试了提取物的抗菌活性,结果表明其对所有受试微生物都具有非常有趣的抗菌谱。由于其非常宜人的香气和味道特性以及生物活性,卡伊纳里草本茶可进一步被推荐为功能性饮料。