全血和提取 DNA 在不同储存时间和条件下的全球甲基化谱的稳定性。
Stability of global methylation profiles of whole blood and extracted DNA under different storage durations and conditions.
机构信息
Center of Systems Molecular Medicine, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA.
Department of Critical Care Medicine, Shanghai JiaoTong University affiliated The Sixth People's Hospital, Shanghai, China.
出版信息
Epigenomics. 2018 Jun;10(6):797-811. doi: 10.2217/epi-2018-0025. Epub 2018 Apr 23.
AIM
To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing.
PATIENTS & METHODS: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration.
RESULTS
Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4°C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples.
CONCLUSION
Archived samples, including DNA stored at 4°C for 20 years, are suitable for comparative studies of DNA methylation.
目的
测试在不同储存条件下储存长达 20 年的 DNA 样本是否可用于使用简化代表性亚硫酸氢盐测序的比较甲基化研究。
患者和方法
比较了五组人类血液 DNA 样本（每组 n=5-6）。分组基于抗凝剂的使用以及储存温度和时间。
结果
在所有储存温度下，包括 4°C，储存 20 年的 DNA 或血液样本中特定基因组区域的甲基化谱在所有储存温度下均相似。存档样本中的样本间相似性水平与最近收集的样本没有显著差异。
结论
包括在 4°C 下储存 20 年的 DNA 在内的存档样本适合用于 DNA 甲基化的比较研究。
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