Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
Genomics. 2019 Jul;111(4):744-752. doi: 10.1016/j.ygeno.2018.04.013. Epub 2018 Apr 21.
A new outbreak pest, Athetis lepigone (Möschler) (Lepidoptera: Noctuidae), has caused severe economic loss in maize crops in China. In order to conduct population genetics study with a more polymorphic and scientific mitochondrial marker, we sequenced the complete mitochondrial genomes of 13 different A. lepigone individuals. Intraspecific comparison of all PCGs showed that the NADH dehydrogenase and cytochrome b genes had the highest nucleotide diversity. We also found evidence of episodic positive selection on two amino acids, which are encoded by NADH dehydrogenase genes (ND3 and ND4L), against a background of widespread neutral selection of all other mitochondrial PCGs. The genetic divergence observed in this study indicated that the cytochrome b gene (CYTB) is better than COI at recovering population structure. The preliminary population genetic analysis illustrated strong gene flow among A. lepigone populations in China. Our study provides basic information for further research on population genetics of A. lepigone.
一种新的爆发性害虫，稻绿蝽（Athetis lepigone）（鳞翅目：夜蛾科），已在中国的玉米作物中造成严重的经济损失。为了用更具多态性和科学性的线粒体标记进行种群遗传学研究，我们对 13 个不同的稻绿蝽个体的完整线粒体基因组进行了测序。所有 PCGs 的种内比较表明，NADH 脱氢酶和细胞色素 b 基因具有最高的核苷酸多样性。我们还发现了两个氨基酸（由 NADH 脱氢酶基因（ND3 和 ND4L）编码）经历间歇性正选择的证据，而其他所有线粒体 PCGs 则受到广泛的中性选择。本研究观察到的遗传分化表明，细胞色素 b 基因（CYTB）比 COI 更能恢复种群结构。初步的种群遗传分析表明，中国稻绿蝽种群之间存在强烈的基因流动。我们的研究为进一步研究稻绿蝽的种群遗传学提供了基础信息。
