印度田间分离株布鲁氏菌 2007BM/1 株的基因组草图序列
Draft genome sequence of field isolate Brucella melitensis strain 2007BM/1 from India.
机构信息
Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India.
Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India.
出版信息
J Glob Antimicrob Resist. 2018 Jun;13:152-153. doi: 10.1016/j.jgar.2018.04.008. Epub 2018 Apr 21.
OBJECTIVES
Brucellosis is among one of the most widespread important global zoonotic diseases that is endemic in many parts of India. Brucella melitensis is supposed to be the most pathogenic species for humans. Here we report the draft genome sequence of B. melitensis strain 2007BM/1 isolated from a human in India.
METHODS
Genomic DNA was extracted from Brucella culture and was sequenced using an Illumina MiSeq platform. The generated reads were assembled using three de novo assemblers and the draft genome was annotated.
RESULTS
This monoisolate, with a genome length of 3268756bp, was found to be resistant to azithromycin and trimethoprim/sulfamethoxazole but susceptible to tetracycline, ofloxacin, rifampicin, ciprofloxacin and doxycycline. The presence of virulence genes in the strain was identified.
CONCLUSIONS
The results obtained will help in understanding drug resistance mechanisms and virulence factors in highly zoonotic B. melitensis and suggest the need for judicious use of antibiotics in livestock health and management practices.
目的
布鲁氏菌病是全球广泛流行的重要动物源性传染病之一，在印度许多地区流行。牛种布鲁氏菌被认为对人类具有最强的致病性。本研究报告了从印度一名患者中分离的牛种布鲁氏菌 2007BM/1 株的基因组序列草案。
方法
从布鲁氏菌培养物中提取基因组 DNA，使用 Illumina MiSeq 平台进行测序。使用三种从头组装程序对生成的reads 进行组装，并对基因组草图进行注释。
结果
该单分离株的基因组大小为 3268756bp，对阿奇霉素和甲氧苄啶/磺胺甲恶唑耐药，但对四环素、氧氟沙星、利福平、环丙沙星和强力霉素敏感。该菌株中存在毒力基因。
结论
研究结果有助于理解高度人畜共患的牛种布鲁氏菌的耐药机制和毒力因子，并提示在牲畜健康和管理实践中需要合理使用抗生素。
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