State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
Liaoning Province Center for Disease Control and Prevention, Shenyang, People's Republic of China.
Emerg Microbes Infect. 2020 Dec;9(1):2147-2156. doi: 10.1080/22221751.2020.1824549.
spp. can cause the zoonosis brucellosis, which affects public health and safety and even economic development. has a smooth phenotype, while 28 isolates had a rough phenotype in 2018. In this study, rough phenotype detection and whole genome sequencing methods were used to analyze the genetic features of rough . A drug susceptibility test was also performed. The results showed that the rough strains originated from strains isolated in China rather than from foreign strains. Furthermore, an MS tree showed that 9 complexes to be epidemic in China. For the rough strains, expression of the metabolic function genes varied in the earlier stages of evolution compared to the cellular process and signalling function genes. Expression of some transcriptional regulatory factors also varied in the later stages of evolution, and compared to MFS transporter genes, ABC transporter genes varied in the earlier stages. Moreover, as there was no significant difference in rifampicin, doxycycline and streptomycin susceptibility between the smooth and rough strains, treatment of brucellosis was not affected by strain type. This study provided important information for understanding the genetics and evolution of rough in China.
种可能导致人畜共患病布鲁氏菌病，这影响公共卫生和安全，甚至经济发展。有光滑的表型，而 28 株在 2018 年具有粗糙的表型。在这项研究中，采用粗糙表型检测和全基因组测序方法分析粗糙的遗传特征。还进行了药敏试验。结果表明，粗糙株来源于中国分离株，而不是来自国外株。此外，MS 树显示 9 个复合体在中国流行。对于粗糙株，与细胞过程和信号功能基因相比，代谢功能基因在进化的早期阶段表达有所不同。一些转录调节因子的表达在进化的后期阶段也有所不同，与 MFS 转运体基因相比，ABC 转运体基因在进化的早期阶段有所不同。此外，由于光滑和粗糙株对利福平、强力霉素和链霉素的敏感性没有差异，因此菌株类型不会影响布鲁氏菌病的治疗。本研究为了解中国粗糙株的遗传学和进化提供了重要信息。
