School of Basic Medical Science & Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical University, Xi'an 710021, China.
Shaanxi Provincial People's Hospital Affiliated to Xi'an Medical University, Xi'an 710068, China.
Med Hypotheses. 2018 Jun;115:1-4. doi: 10.1016/j.mehy.2018.03.005. Epub 2018 Mar 20.
Regulatory T cell (Treg cell) is an important immunosuppressive T cell subset and plays a dominant role in maintaining the immune balance in vivo. The function defects in Treg cells have been involved in the pathogenesis of many autoimmune diseases. The detection of Treg cell suppressive function is important for early diagnosis and prediction of response to treatment for autoimmune diseases. The traditional detection of Treg cell suppressive function needs at least 20 mL peripheral blood sample of patients and the results would be got in sixth day, therefore, it could not be widely applied in clinical. However, to find fast and simple detection method is very important. CD147 is a transmembrane protein and its expression is related to Treg cell suppressive function. Recent research has shown that the Treg cells with high CD147 expression have stronger suppressive function than which with low CD147 expression. In this work, we detected the ratio of CD147/CD147 in CD4CD25 T cells in patients with active AS using fluorescence-activated cell sorter (FACS). The results show the ratio of CD147/CD147 decreased obviously in patients with active AS compared with healthy controls, which reflects the suppressive function deficit of Treg cell. In the same time, the detection of the ratio of CD147/CD147 needs only 150 μL peripheral blood sample and the result would be got in 4 h. We therefore hypothesize that the ratio of CD147/CD147 is a good indicator for the Treg cell function, and it is especially suitable for early diagnosis and prediction of response to therapy targeted recovering Treg cell function in autoimmune diseases.
调节性 T 细胞（Treg 细胞）是一种重要的免疫抑制性 T 细胞亚群，在体内维持免疫平衡中发挥主导作用。Treg 细胞功能缺陷与许多自身免疫性疾病的发病机制有关。Treg 细胞抑制功能的检测对于自身免疫性疾病的早期诊断和预测治疗反应具有重要意义。传统的 Treg 细胞抑制功能检测需要至少 20ml 患者外周血样本，结果需要 6 天才能获得，因此无法广泛应用于临床。然而，寻找快速、简单的检测方法非常重要。CD147 是一种跨膜蛋白，其表达与 Treg 细胞抑制功能有关。最近的研究表明，高表达 CD147 的 Treg 细胞比低表达 CD147 的 Treg 细胞具有更强的抑制功能。在这项工作中，我们使用流式细胞术（FACS）检测了活动期 AS 患者外周血中 CD4+CD25+T 细胞中 CD147/CD147 的比值。结果显示，活动期 AS 患者 CD147/CD147 比值明显低于健康对照组，反映了 Treg 细胞抑制功能的缺陷。同时，CD147/CD147 的检测只需 150μl 外周血样本，4 小时即可获得结果。因此，我们假设 CD147/CD147 的比值是 Treg 细胞功能的一个良好指标，尤其适用于自身免疫性疾病中早期诊断和预测靶向恢复 Treg 细胞功能的治疗反应。
