脊椎动物红细胞中的 7SL RNA。
7SL RNA in vertebrate red blood cells.
机构信息
Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland 21218, USA.
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
出版信息
RNA. 2018 Jul;24(7):908-914. doi: 10.1261/rna.065474.117. Epub 2018 Apr 23.
We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates from a lysate of RBCs with a number of membrane-binding proteins. Human and mouse RBCs also contain a previously undescribed 68 nt RNA, sRN7SL, derived from the "S domain" of 7SL RNA. We discuss the possibility that 7SL RNA is selectively protected from nucleases by association with the RBC membrane. Because 7SL is not associated with the canonical proteins of the SRP, it could represent a nonfunctional remnant of the protein synthetic machinery. Alternatively, it could play a new, as yet undefined role in RBC metabolism.
我们报告称，信号识别颗粒（SRP）的 RNA 成分 7SL，是人类、小鼠和青蛙成熟红细胞（RBC）中丰富的非编码 RNA（ncRNA）。RBC 中的 7SL RNA 不与 SRP 的典型蛋白相关联。相反，它与从 RBC 裂解物中沉淀下来的许多膜结合蛋白一起共沉淀。人和小鼠 RBC 中还含有一种以前未描述的 68nt RNA，sRN7SL，来自 7SL RNA 的“ S 结构域”。我们讨论了 7SL RNA 通过与 RBC 膜结合而被选择性地免受核酸酶侵害的可能性。因为 7SL 不与 SRP 的典型蛋白相关联，所以它可能代表蛋白质合成机制的无功能残余物。或者，它可以在 RBC 代谢中发挥新的、尚未定义的作用。
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