鉴定与结核分枝杆菌吡嗪酸/吡嗪酰胺耐药相关的 LprG () 、 、 、 、 和 中的新型突变。
Identification of Novel Mutations in LprG (), , , , , and Associated with Pyrazinoic Acid/Pyrazinamide Resistance in Mycobacterium tuberculosis.
机构信息
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA.
Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
出版信息
Antimicrob Agents Chemother. 2018 Jun 26;62(7). doi: 10.1128/AAC.00430-18. Print 2018 Jul.
Abstract
Despite progress, the mechanisms of action and resistance of pyrazinamide (PZA) are not well understood. We characterized 109 mutants resistant to pyrazinoic acid (POA), the active form of PZA, and found that while most ( = 101 [93%]) mutants had mutations and 4 (4%) had mutations (S91G), new mutations in () and ( = 4 [4%]), , , , and (cytochrome P450 128) were identified, shedding new light on the mechanisms of action and resistance of PZA in .
摘要
尽管取得了进展，但吡嗪酰胺（PZA）的作用机制和耐药机制仍不清楚。我们对 109 株耐吡嗪酸（POA）的突变体进行了特征描述，POA 是 PZA 的活性形式，结果发现，虽然大多数（=101 [93%]）突变株具有 突变，而 4 株（4%）具有 突变（S91G），但在 （=4 [4%]）中发现了新的突变， （细胞色素 P450 128），这为 PZA 在结核分枝杆菌中的作用机制和耐药机制提供了新的认识。
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