Majlesi Amir, Kakhki Reza Kamali, Mozaffari Nejad Amir Sasan, Mashouf Rasoul Yousefi, Roointan Amir, Abazari Malek, Alikhani Mohammad Yousef
Department of Gastroenterology, Hamadan University of Medical Sciences, Hamadan, Iran.
Department Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran.
Saudi J Biol Sci. 2018 Mar;25(3):426-430. doi: 10.1016/j.sjbs.2016.11.019. Epub 2016 Dec 19.
Plasmid mediated quinolone resistance (PMQR) determinants have arisen as a significant concern in recent years. The aim of this study was screening of resistant-clinical isolates to fluoroquinolone antibiotics and detection of and genes. For this purpose we collected 100 fluoroquinolone-resistant which were from 3 hospitals in Hamadan, west provinces of Iran, between October 2012 and June 2013. The all samples were identified by biochemical tests and confirmed by PCR method. Antimicrobial susceptibility to 14 antimicrobial agents including levofloxacin and ciprofloxacin were determined by disk diffusion methods and ciprofloxacin MIC was obtained by broth microdilution method as Clinical Laboratory Standards Institute (CLSI) recommendations. The isolates were screened for the presence of , , and genes using PCR assay. Among the screened isolates, 64 strains (64%) of , 23 strains (23%) of , 13 strains (13%) of were collected as quinolone-resistant isolates. out of 100 isolates, two (2%) were positive for , seventeen (17%) isolates were positive for and we did not find gene in any of the isolates. There were also 32 positive isolates for determinant. We described the prevalence of and genes in fluoroquinolone-resistant in Hamadan city. The carriage rate of multidrug-resistant in healthy people in Hamadan City is extremely high. Moreover, genes encoding transferable quinolones, in particular , are highly prevalent in these strains.
质粒介导的喹诺酮耐药性（PMQR）决定因素近年来已成为一个重大问题。本研究的目的是筛选对氟喹诺酮类抗生素耐药的临床分离株，并检测[具体基因名称1]和[具体基因名称2]基因。为此，我们收集了2012年10月至2013年6月期间来自伊朗西部哈马丹市3家医院的100株氟喹诺酮耐药[细菌名称]。所有样本均通过生化试验进行鉴定，并通过PCR方法进行确认。采用纸片扩散法测定对包括左氧氟沙星和环丙沙星在内的14种抗菌药物的药敏情况，并按照临床实验室标准协会（CLSI）的建议，通过肉汤微量稀释法获得环丙沙星的最低抑菌浓度（MIC）。使用PCR检测法筛选分离株中[具体基因名称1]、[具体基因名称2]、[具体基因名称3]和[具体基因名称4]基因的存在情况。在筛选出的分离株中，收集到64株（64%）[具体基因名称1]、23株（23%）[具体基因名称2]、13株（13%）[具体基因名称3]作为喹诺酮耐药分离株。在100株分离株中，2株（2%）[具体基因名称5]呈阳性，17株（17%）分离株[具体基因名称6]呈阳性，且在任何分离株中均未发现[具体基因名称7]基因。此外，有32株分离株对[耐药决定因素名称]呈阳性。我们描述了哈马丹市氟喹诺酮耐药[细菌名称]中[具体基因名称1]和[具体基因名称2]基因的流行情况。哈马丹市健康人群中多重耐药[细菌名称]的携带率极高。此外，编码可转移喹诺酮的基因，特别是[具体基因名称1]，在这些菌株中高度流行。
