Department of Chemistry and Biochemistry , University of Bern , Freiestrasse 3 , CH-3012 Bern , Switzerland.
Bioconjug Chem. 2018 May 16;29(5):1505-1509. doi: 10.1021/acs.bioconjchem.8b00263. Epub 2018 Apr 26.
Vesicle-shaped supramolecular polymers are formed by self-assembly of a DNA duplex containing phenanthrene overhangs at both ends. In the presence of spermine, the phenanthrene overhangs act as sticky ends linking the DNA duplexes together. In aqueous solution, the assembly leads to vesicles with a diameter in the range of 50-200 nm. Fluorescence measurements show that the assembled phenanthrene units act as light-harvesting complexes and transfer absorbed energy to an acceptor, such as pyrene or Cy3, which can either be directly added to the polymer or attached via a complementary DNA strand. The presence of DNA in the nanostructures allows the construction of light-harvesting vesicles that are amenable to derivatization with different functional groups.
囊泡状超分子聚合物是由一条两端带有菲并芘侧链的 DNA 双链自组装而成。在 spermine 的存在下，菲并芘侧链作为粘性末端将 DNA 双链连接在一起。在水溶液中，组装会导致形成直径在 50-200nm 范围内的囊泡。荧光测量表明，组装的菲并芘单元可以作为光捕获复合物，并将吸收的能量传递给一个受体，如芘或 Cy3，后者可以直接添加到聚合物中，也可以通过互补的 DNA 链连接到聚合物上。纳米结构中 DNA 的存在允许构建光捕获囊泡，这些囊泡可以用不同的官能团进行衍生化。
