白纹伊蚊发育过程中与蜕皮相关的多效性神经肽表达
Ecdysis-related pleiotropic neuropeptides expression during Anopheles albimanus development.
作者信息
Alvarado-Delgado Alejandro, Moran-Francia Ken, Perales-Ortiz Guillermo, Rodríguez Mario Henry, Lanz-Mendoza Humberto
机构信息
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública. Cuernavaca, Morelos, México.
出版信息
Salud Publica Mex. 2018 Jan-Feb;60(1):48-55. doi: 10.21149/8134.
OBJECTIVE
To analyze the transcription pattern of neuropeptides in the ontogeny of a malaria vector, the mosquito Anopheles albimanus.
MATERIALS AND METHODS
The transcription pattern of Crustacean CardioActive peptide (CCAP), corazonin, Ecdysis Triggering Hormone (ETH), allatostatin-A, orcokinin, Insulin Like Peptide 2 (ILP2), Insulin Like Peptide 5 (ILP5) and bursicon was evaluated using qPCR on larvae (1st - 4th instar), pupae and adult mosquitoes.
RESULTS
Unlike in other insects, transcripts of CCAP (70.8%), ETH (60.2%) and corazonin (76.5%) were expressed in 4th instar larvae, probably because these three neuropeptides are associated with the beginning of ecdysis. The neuropeptide ILP2 showed higher transcription levels in other stages and orcokinin decreased during the development of the mosquito.
CONCLUSIONS
The CCAP, corazonin and ETH neuropeptidesare potential targets for the design of control strategies aimed at disrupting An. albiamnus larval development.
目的
分析疟蚊媒介白纹伊蚊个体发育过程中神经肽的转录模式。
材料与方法
采用定量聚合酶链反应（qPCR）对幼虫（一龄至四龄）、蛹和成年蚊子中甲壳动物心脏活性肽（CCAP）、促心肽、蜕皮触发激素（ETH）、抑咽侧体素-A、咽侧体激肽、胰岛素样肽2（ILP2）、胰岛素样肽5（ILP5）和羽化激素的转录模式进行评估。
结果
与其他昆虫不同，CCAP（70.8%）、ETH（60.2%）和促心肽（76.5%）的转录本在四龄幼虫中表达，可能是因为这三种神经肽与蜕皮开始有关。神经肽ILP2在其他阶段表现出较高的转录水平，而咽侧体激肽在蚊子发育过程中减少。
结论
CCAP、促心肽和ETH神经肽是旨在破坏白纹伊蚊幼虫发育的控制策略设计的潜在靶点。
Zotero 插件