Alvarado-Delgado Alejandro, Martínez-Barnetche Jesús, Téllez-Sosa Juan, Rodríguez Mario H, Gutiérrez-Millán Everardo, Zumaya-Estrada Federico A, Saldaña-Navor Vianey, Rodríguez María Carmen, Tello-López Ángel, Lanz-Mendoza Humberto
Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad 655, Santa María Ahuacatitlán, C.P. 62100 Cuernavaca, Morelos, Mexico.
Curr Res Insect Sci. 2021 Apr 22;1:100014. doi: 10.1016/j.cris.2021.100014. eCollection 2021.
Insect neuropeptides, play a central role in the control of many physiological processes. Based on an analysis of brain transcriptome a neuropeptide precursor database of the mosquito was described. Also, we observed that adipokinetic hormone/corazonin-related peptide (ACP), hugin and corazonin encoding genes were differentially expressed during infection. Transcriptomic data from brain identified 29 pre-propeptides deduced from the sequences that allowed the prediction of at least 60 neuropeptides. The predicted peptides include isoforms of allatostatin C, orcokinin, corazonin, adipokinetic hormone (AKH), SIFamide, capa, hugin, pigment-dispersing factor, adipokinetic hormone/corazonin-related peptide (ACP), tachykinin-related peptide, trissin, neuropeptide F, diuretic hormone 31, bursicon, crustacean cardioactive peptide (CCAP), allatotropin, allatostatin A, ecdysis triggering hormone (ETH), diuretic hormone 44 (Dh44), insulin-like peptides (ILPs) and eclosion hormone (EH). The analysis of the genome of and the generated transcriptome, provided evidence for the identification of myosuppressin neuropeptide precursor. A quantitative analysis documented increased expression of precursors encoding ACP peptide, hugin and corazonin in the mosquito brain after infection. This work represents an initial effort to characterize the neuropeptide precursors repertoire of and provides information for understanding neuroregulation of the mosquito response during infection.
昆虫神经肽在许多生理过程的控制中起着核心作用。基于对大脑转录组的分析，描述了蚊子的神经肽前体数据库。此外，我们观察到脂肪动激素/心钠素相关肽（ACP）、hugin和心钠素编码基因在感染期间差异表达。来自大脑的转录组数据确定了从序列推导的29种前原肽，这些序列可预测至少60种神经肽。预测的肽包括抑咽侧体素C、促心搏肽、心钠素、脂肪动激素（AKH）、SIF酰胺、capa、hugin、色素分散因子、脂肪动激素/心钠素相关肽（ACP）、速激肽相关肽、trissin、神经肽F、利尿激素31、bursicon、甲壳动物心脏活性肽（CCAP）、促咽侧体素、抑咽侧体素A、蜕皮触发激素（ETH）、利尿激素44（Dh44）、胰岛素样肽（ILP）和羽化激素（EH）的亚型。对[蚊子名称未给出]基因组和生成的转录组的分析，为肌抑制素神经肽前体的鉴定提供了证据。定量分析表明，感染后蚊子大脑中编码ACP肽、hugin和心钠素的前体表达增加。这项工作是对[蚊子名称未给出]神经肽前体库进行表征的初步努力，并为理解蚊子在感染期间反应的神经调节提供了信息。
