Antwi-Boasiako Charles, Donkor Eric S, Sey Fredericka, Dzudzor Bartholomew, Dankwah Gifty B, Otu Kate H, Doku Alfred, Dale Campbell A, Ekem Ivy
Department of Physiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana.
Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana.
Diseases. 2018 Apr 21;6(2):29. doi: 10.3390/diseases6020029.
Soluble adhesion molecules are involved in the gathering and joining of inflammatory cells to vascular endothelium. Therefore, they serve as potential markers of endothelial dysfunction in vascular diseases including sickle cell disease (SCD). In Ghana, there are scarcely any report on the levels of adhesion molecules among SCD patients. The current study aimed to determine plasma levels of ICAM-1, VCAM-1 and E-Selectin as markers of endothelial dysfunction in SCD patients in steady state, complications and controls.
This was a cross-sectional study involving 60 HbAA controls, 46 HbSS steady state, 57 HbSS VOC, 18 HbSC VOC, 21 HbSS with leg ulcer and 11 HbSS with priapism. Blood samples were collected from all the study subjects ( = 213) and processed into plasma. The plasma levels of VCAM-1, ICAM-1 and E-Selectin concentrations of SCD patients and controls were measured using a double sandwich ELISA technique. Demographic information was also collected from the study subjects.
Levels of all soluble proteins (ICAM-1, VCAM-1 and E-Selectin) were significantly higher in HbSS steady-state patients compared to non-SCD controls ( < 0.001). Generally, SCD patients with complications had relatively higher levels of the soluble proteins compared to those in the steady-state. Of the SCD patients with complications, those with vaso-occlusion crisis (HbSS VOC) had relatively higher levels of ICAM-1, VCAM-1 and E-Selectin at (62.42 ng/mL ± 26.09), (634.99 ng/mL ± 324.31) and (236.77 ng/mL ± 114.40) respectively; Conclusion: Although levels of adhesion molecules were high in all the SCD patients with complications, those with vaso-occlusive crisis had higher levels. This might reflect an ongoing endothelial dysfunction in these patients. SCD patients with vaso-occlusive crisis presents with a more severe pathophysiology condition.
可溶性黏附分子参与炎症细胞与血管内皮的聚集和结合。因此，它们可作为包括镰状细胞病（SCD）在内的血管疾病中内皮功能障碍的潜在标志物。在加纳，几乎没有关于SCD患者黏附分子水平的报道。本研究旨在测定处于稳定状态、有并发症的SCD患者以及对照组中细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和E-选择素的血浆水平，作为内皮功能障碍的标志物。
这是一项横断面研究，涉及60名HbAA对照组、46名HbSS稳定状态患者、57名HbSS血管闭塞性危机（VOC）患者、18名HbSC VOC患者、21名患有腿部溃疡的HbSS患者和11名患有阴茎异常勃起的HbSS患者。从所有研究对象（n = 213）采集血样并处理成血浆。采用双夹心酶联免疫吸附测定（ELISA）技术测量SCD患者和对照组的血浆中VCAM-1、ICAM-1和E-选择素的浓度。还从研究对象处收集了人口统计学信息。
与非SCD对照组相比，HbSS稳定状态患者中所有可溶性蛋白（ICAM-1、VCAM-1和E-选择素）的水平均显著更高（P < 0.001）。一般来说，有并发症的SCD患者的可溶性蛋白水平比稳定状态的患者相对更高。在有并发症的SCD患者中，患有血管闭塞性危机（HbSS VOC）的患者的ICAM-1、VCAM-1和E-选择素水平相对更高，分别为（62.42 ng/mL ± 26.09）、（634.99 ng/mL ± 324.31）和（236.77 ng/mL ± 114.40）。结论：虽然所有有并发症的SCD患者的黏附分子水平都很高，但患有血管闭塞性危机的患者水平更高。这可能反映了这些患者中持续存在的内皮功能障碍。患有血管闭塞性危机的SCD患者表现出更严重的病理生理状况。
