Center for Post-Doctoral Research, Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China; Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China.
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.
Carbohydr Polym. 2018 Jul 15;192:143-149. doi: 10.1016/j.carbpol.2018.03.061. Epub 2018 Mar 19.
In this study, BCPS-1, a polysaccharide previously isolated and characterized from Bupleurum chinense was chemically modified to yield two sulfated derivatives, which we designated as S-BCP and S-BCP. The physicochemical properties of these sulfated derivatives were then determined by high performance liquid chromatography (HPLC), Fourier transform infrared spectrometry (FT-IR), and gas chromatography (GC), and then compared with those of BCPS-1. Furthermore, the antioxidant activities of all three polysaccharides were also evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, superoxide radical assay and hydroxyl radical assay, while their effects against HO-induced cellular senescence were determined using senescence-associated β-galactosidase (SA-β-gal) staining, cell cycle assay and immunoblotting in HO-induced mouse lung endothelial cells (MLECs). Compared to BCPS-1, S-BCP and S-BCP exhibited remarkable antioxidant effect, and in a concentration-dependent manner. They also provided stronger protection against HO-induced cellular senescence in MLECs. These results indicated that the sulfate group in the modified B. chinense polysaccharides might play an important role in radical scavenging and resistance to HO-induced senescence. These sulfated polysaccharides could be considered as novel pharmaceutical products with potential antioxidant and anti-senescence effects.
在这项研究中，我们对先前从柴胡中分离和鉴定的多糖 BCPS-1 进行了化学修饰，得到了两种硫酸化衍生物，我们将其命名为 S-BCP 和 S-BCP。然后通过高效液相色谱（HPLC）、傅里叶变换红外光谱（FT-IR）和气相色谱（GC）测定了这些硫酸化衍生物的理化性质，并与 BCPS-1 进行了比较。此外，还使用 1,1-二苯基-2-苦基肼（DPPH）自由基测定法、超氧自由基测定法和羟基自由基测定法评估了这三种多糖的抗氧化活性，并用衰老相关β-半乳糖苷酶（SA-β-gal）染色、细胞周期测定法和免疫印迹法测定了它们对 HO 诱导的细胞衰老的抑制作用。与 BCPS-1 相比，S-BCP 和 S-BCP 表现出显著的抗氧化作用，并呈浓度依赖性。它们还能更强地抵抗 HO 诱导的 MLECs 细胞衰老。这些结果表明，修饰后的柴胡多糖中的硫酸基团可能在清除自由基和抵抗 HO 诱导的衰老方面发挥重要作用。这些硫酸化多糖可被视为具有潜在抗氧化和抗衰老作用的新型药物。
