Guna Serrano María Remedio, Larrosa Escartín Nieves, Marín Arriaza Mercedes, Rodríguez Díaz Juan Carlos
Servicio de Microbiología, Consorcio Hospital General Universitario de Valencia, Valencia, España.
Servicio de Microbiología, Hospital Universitario Vall d'Hebron, Barcelona, España.
Enferm Infecc Microbiol Clin (Engl Ed). 2019 May;37(5):335-340. doi: 10.1016/j.eimc.2018.03.005. Epub 2018 Apr 22.
In this review we try to update the new procedures applicable in the microbiological diagnosis of bacteriemia and fungemias.
Review of scientific literature.
After defining the process and indicating its fundamental principles, the main biomarkers used in clinical practice are reviewed. Subsequently, the particularities of the pre-analytical phase (collection and transport of samples) are highlighted and the steps to follow for the microbiological identification by classical methods are detailed, based on the culture of the blood samples. In the following section, we review the diagnostic methods not culture based, including those that detect the presence of the genome of the microorganism and those based on the study of proteome by mass spectrometry. The last section describes the procedures more frecuently used for the study of antibiotic susceptibility, both by phenotypic and genotypic methods.
在本综述中，我们试图更新适用于菌血症和真菌血症微生物诊断的新程序。
对科学文献进行综述。
在定义该过程并指出其基本原则后，对临床实践中使用的主要生物标志物进行了综述。随后，强调了分析前阶段（样本采集和运输）的特殊性，并详细介绍了基于血样培养通过经典方法进行微生物鉴定的步骤。在接下来的部分中，我们回顾了非基于培养的诊断方法，包括那些检测微生物基因组存在的方法以及基于质谱蛋白质组学研究的方法。最后一部分描述了通过表型和基因型方法更常用于研究抗生素敏感性的程序。
