Golovatskiĭ I D, Tsegel'skiĭ A A
Ukr Biokhim Zh (1978). 1988 Mar-Apr;60(2):30-5.
The interrelationship of NAD and adenosine (inosine) conversions in the rat liver is investigated. The ratio of products of NAD+ conversions (ADP-ribose, inosine, hypoxanthine and ribose phosphates) are established. AMP and adenosine are not detected, which indicates an availability of different activities of the corresponding enzymes. It is shown that under conditions of the high inorganic phosphate concentration (33 mM) ribose-1-phosphate, formed in the purine nucleoside phosphorylase reaction, is accumulated due to the phosphoribomutase inhibition, but in the presence of NAD+ the utilization of ribose phosphate increases significantly. Nicotinamide inhibits the NAD+-glycohydrolase reaction in the system containing 33 mM phosphate, NAD+ and adenosine and simultaneously it lowers the utilization of ribose.
研究了大鼠肝脏中NAD与腺苷（肌苷）转化的相互关系。确定了NAD⁺转化产物（ADP-核糖、肌苷、次黄嘌呤和核糖磷酸）的比例。未检测到AMP和腺苷，这表明相应酶具有不同活性。结果表明，在高无机磷酸盐浓度（33 mM）条件下，嘌呤核苷磷酸化酶反应中形成的核糖-1-磷酸由于磷酸核糖变位酶的抑制而积累，但在存在NAD⁺的情况下，核糖磷酸的利用率显著增加。烟酰胺在含有33 mM磷酸盐、NAD⁺和腺苷的体系中抑制NAD⁺-糖水解酶反应，同时降低核糖的利用率。
