Wu Cheng Li, Lin Xin da, Jiang Yan Yun, Meng Shuai Shuai
College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Ying Yong Sheng Tai Xue Bao. 2018 Feb;29(2):659-668. doi: 10.13287/j.1001-9332.201802.032.
Curly wing is one of the most frequently used genetic markers in Drosophila melanogaster, but its molecular mechanism is remained unclear. Previous results have showed that physicochemical stimulation would affect the formation of the cruly wing. Our recent study found that HO could not only affect the eclosion rate of D. melanogaster, but also induce the formation of curly wing. Here, we aimed to uncover the specific factors influencing the formation of curly wing in D. melanogaster via changing the concentration of HO and the temperature as well as the time of HO treatment. We measured the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX), in order to examine the effects of HO on antioxidative capacity of D. melanogaster. The results showed that the eclosion rate of D. melanogaster was inversely correlated with the concentration of HO. The change of temperature, HO concentration and the period of HO treatment affected the degree of the curl and the proportion of the curly wing. The white mutant flies responded most significantly to these three conditions, the mini-white (white gene reverse mutation) flies could rescue the curly phenotype, and responded similarly to the wild type OR. HO had effects on the formation of the curly wing which contained the Cy mutation, leading to increased rate of the curly wing. D. melanogaster treated with HO would reduce the antioxidative capacity. Results from real-time quantitative PCR showed that HO treatment resulted in a change in gene expression. The formation of curly wing was a complicated process, and HO might act as a signaling molecule or indirectly affect certain factors in the formation of curly wing in D. melano-gaster. This process might share the same signaling pathway with the Cy mutant, or might be regulated by different modulating patterns.
卷翅是黑腹果蝇中最常用的遗传标记之一，但其分子机制仍不清楚。先前的结果表明，物理化学刺激会影响卷翅的形成。我们最近的研究发现，过氧化氢不仅会影响黑腹果蝇的羽化率，还会诱导卷翅的形成。在此，我们旨在通过改变过氧化氢的浓度、温度以及过氧化氢处理的时间，来揭示影响黑腹果蝇卷翅形成的具体因素。我们测量了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-PX）的活性，以检测过氧化氢对黑腹果蝇抗氧化能力的影响。结果表明，黑腹果蝇的羽化率与过氧化氢的浓度呈负相关。温度、过氧化氢浓度和过氧化氢处理时间的变化会影响卷翅的卷曲程度和卷翅的比例。白眼突变果蝇对这三个条件的反应最为显著，小白眼（白眼基因反向突变）果蝇可以挽救卷曲表型，并且其反应与野生型OR相似。过氧化氢对含有卷翅突变（Cy）的卷翅形成有影响，导致卷翅率增加。用过氧化氢处理的黑腹果蝇会降低其抗氧化能力。实时定量PCR结果表明，过氧化氢处理导致基因表达发生变化。卷翅的形成是一个复杂的过程，过氧化氢可能作为一种信号分子，或间接影响黑腹果蝇卷翅形成中的某些因素。这个过程可能与卷翅突变体共享相同的信号通路，或者可能受不同的调控模式调节。
