Humphries D E, Silbert J E
Connective Tissue Laboratory, Veterans Administration Outpatient Clinic, Boston, MA 02108.
Biochem Biophys Res Commun. 1988 Jul 15;154(1):365-71. doi: 10.1016/0006-291x(88)90694-8.
Bovine aorta endothelial cells were cultured in medium containing [3H]glucosamine, [35S]sulfate, and various concentrations of chlorate. Cell growth was not affected by 10 mM chlorate, while 30 mM chlorate had a slight inhibitory effect. Chlorate concentrations greater than 10 mM resulted in significant undersulfation of chondroitin. With 30 mM chlorate, sulfation of chondroitin was reduced to 10% and heparan to 35% of controls, but [3H]glucosamine incorporation on a per cell basis did not appear to be inhibited. Removal of chlorate from the culture medium of cells resulted in the rapid resumption of sulfation.
将牛主动脉内皮细胞培养在含有[3H]葡糖胺、[35S]硫酸盐和不同浓度氯酸盐的培养基中。10 mM氯酸盐不影响细胞生长，而30 mM氯酸盐有轻微抑制作用。氯酸盐浓度大于10 mM会导致硫酸软骨素显著硫酸化不足。在30 mM氯酸盐作用下，硫酸软骨素的硫酸化降至对照的10%，硫酸乙酰肝素降至35%，但基于每个细胞的[3H]葡糖胺掺入似乎未受抑制。从细胞培养基中去除氯酸盐后，硫酸化迅速恢复。
